| Literature DB >> 1884974 |
Y Lautrou1, P Rainard, B Poutrel, M S Zygmunt, A Vénien, J Dufrenoy.
Abstract
The protein X of Steptococcus agalactiae is a surface antigen included in the typing scheme of group B streptococci (GBS). We have developed a monoclonal antibody to the protein X and used it to purify this antigen by affinity chromatography. Electrophoresis in polyacrylamide, and immunoblotting using the monoclonal antibody or a rabbit antiserum raised with the affinity purified protein X, revealed a major band in the region of 200 kDa and a smaller one at 100 kDa. The isolated protein X will make possible investigations of its potential role in virulence and protection.Entities:
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Year: 1991 PMID： 1884974 DOI： 10.1016/0378-1097(91)90584-w
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742