| Literature DB >> 18848515 |
Jinfu Zhao1, Bryan A Bertoglio, Michael J Devinney, Kirk E Dineley, Alan R Kay.
Abstract
Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport Green with all the cationic transition metals found within cells, Cr, Mn, Fe, Co, and Cu, as well as Ni and Cd, by measuring their dissociation constants. In addition, we have shown how FluoZin-3 can be used to quantify the concentration of copper in a cell-free assay and report that the fluorescence of Newport Green is boosted by both Cu(I) and Fe(II). Furthermore, we have introduced diagnostics for detecting the interference of metals other than zinc with its measurement within cells.Entities:
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Year: 2008 PMID： 18848515 PMCID： PMC2630397 DOI： 10.1016/j.ab.2008.09.019
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365