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Literature DB >> 18846397

Production and partial purification of alpha-amylase from a novel isolate Streptomyces gulbargensis.

Dastager G Syed¹, Dayanand Agasar, Ashok Pandey.

Abstract

Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45 degrees C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating alpha-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa.

Entities: Chemical Species

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Year: 2008 PMID： 18846397 DOI： 10.1007/s10295-008-0484-9

Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN： 1367-5435 Impact factor: 3.346

19 in total

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