Literature DB >> 1884517

A simplified protocol for fluorescence in situ hybridization with repetitive DNA probes and its use in clinical cytogenetics.

O Bartsch1, E Schwinger.   

Abstract

A method for chromosome-specific staining and its use in clinical cytogenetics is described. This fluorescence in situ hybridization protocol for repetitive DNA probes results in yellow-green fluorescent signals on orange-red stained chromosomes. Special characteristics are its simplicity, the use of digoxigenin-11-dUTP for labeling, and the combination of high stringency criteria for hybridization and low stringency for washing. The method is particularly advantageous in cases with structurally abnormal extra chromosomes (ESACs), marker chromosomes of gonosomal origin, and chromosomal mosaicism. It may also facilitate the screening of cases for fragile X. The chromosome-specific staining can be done within 1 working-day.

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Year:  1991        PMID： 1884517     DOI： 10.1111/j.1399-0004.1991.tb03067.x

Source DB:  PubMed          Journal:  Clin Genet        ISSN： 0009-9163            Impact factor:   4.438


  7 in total

1.  A 20-year-old male with partial trisomy 18q- as diagnosed by in situ hybridization.

Authors:  C H Gravholt; U Friedrich; J Nielsen
Journal:  Hum Genet       Date:  1992-03       Impact factor: 4.132

2.  Prenatal detection of chromosome aneuploidies in uncultured chorionic villus samples by FISH.

Authors:  T Bryndorf; B Christensen; M Vad; J Parner; M P Carelli; B E Ward; K W Klinger; J Bang; J Philip
Journal:  Am J Hum Genet       Date:  1996-10       Impact factor: 11.025

3.  DNA, FISH and complementation studies in ICF syndrome: DNA hypomethylation of repetitive and single copy loci and evidence for a trans acting factor.

Authors:  S Schuffenhauer; O Bartsch; M Stumm; T Buchholz; T Petropoulou; S Kraft; B Belohradsky; G K Hinkel; T Meitinger; R D Wegner
Journal:  Hum Genet       Date:  1995-11       Impact factor: 4.132

4.  FISH detection of trisomy 21 in interphase by the simultaneous use of two differentially labelled cosmid contigs.

Authors:  A F Davies; L Barber; M Murer-Orlando; M Bobrow; M Adinolfi
Journal:  J Med Genet       Date:  1994-09       Impact factor: 6.318

5.  "Compensatory" uniparental disomy of chromosome 21 in two cases.

Authors:  O Bartsch; M B Petersen; I Stuhlmann; G Mau; M Frantzen; E Schwinger; S E Antonarakis; M Mikkelsen
Journal:  J Med Genet       Date:  1994-07       Impact factor: 6.318

6.  Delineation of marker chromosomes by reverse chromosome painting using only a small number of DOP-PCR amplified microdissected chromosomes.

Authors:  R Viersbach; G Schwanitz; M M Nöthen
Journal:  Hum Genet       Date:  1994-06       Impact factor: 4.132

7.  Cytogenetic, FISH and DNA studies in 11 individuals from a family with two siblings with dup(21q) Down syndrome.

Authors:  O Bartsch; U König; M B Petersen; H Poulsen; M Mikkelsen; F Palau; F Prieto; E Schwinger
Journal:  Hum Genet       Date:  1993-09       Impact factor: 4.132
  7 in total

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