Literature DB >> 18839953

Lanthanide-based protease activity sensors for time-resolved fluorescence measurements.

Shin Mizukami1, Kazuhiro Tonai, Masahiro Kaneko, Kazuya Kikuchi.   

Abstract

Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases.

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Year:  2008        PMID： 18839953     DOI： 10.1021/ja800322b

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  6 in total

1.  On the Synthesis of 1,4,7-Tris(tert-butoxycarbonylmethyl)-1,4,7,10-tetraazacyclododecane.

Authors:  Bhumasamudram Jagadish; Gayle L Brickert-Albrecht; Gary S Nichol; Eugene A Mash; Natarajan Raghunand
Journal:  Tetrahedron Lett       Date:  2011-04-27       Impact factor: 2.415

2.  SIB-DOTA: a trifunctional prosthetic group potentially amenable for multi-modal labeling that enhances tumor uptake of internalizing monoclonal antibodies.

Authors:  G Vaidyanathan; B J White; D J Affleck; X G Zhao; P C Welsh; D McDougald; J Choi; M R Zalutsky
Journal:  Bioorg Med Chem       Date:  2012-10-29       Impact factor: 3.641

3.  Visualization of Fluoride Ions In Vivo Using a Gadolinium(III)-Coumarin Complex-Based Fluorescence/MRI Dual-Modal Probe.

Authors:  Yue Wang; Renfeng Song; Huan Feng; Ke Guo; Qingtao Meng; Haijun Chi; Run Zhang; Zhiqiang Zhang
Journal:  Sensors (Basel)       Date:  2016-12-16       Impact factor: 3.576

Review 4.  Luminescent Lifetime Regulation of Lanthanide-Doped Nanoparticles for Biosensing.

Authors:  Mingkai Wang; Chuanyu Hu; Qianqian Su
Journal:  Biosensors (Basel)       Date:  2022-02-19

5.  A highly selective and sensitive chemiluminescent probe for leucine aminopeptidase detection in vitro, in vivo and in human liver cancer tissue.

Authors:  Baoqu Wang; Zhenzhou Chen; Xiaohong Cen; Yuqing Liang; Liyi Tan; En Liang; Lu Zheng; Yanjun Zheng; Zhikun Zhan; Kui Cheng
Journal:  Chem Sci       Date:  2022-01-27       Impact factor: 9.825

6.  Leucine aminopeptidase may contribute to the intrinsic resistance of cancer cells toward cisplatin as revealed by an ultrasensitive fluorescent probe.

Authors:  Qiuyu Gong; Wen Shi; Lihong Li; Huimin Ma
Journal:  Chem Sci       Date:  2015-10-22       Impact factor: 9.825
  6 in total

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