Literature DB >> 18839104

Multiplex ABO genotyping by minisequencing.

Gianmarco Ferri1, Susi Pelotti.   

Abstract

The minisequencing multiplex reaction to genotype ABO blood group system is described and discussed. This method was found to be a reproducible strategy to type 6 common alleles (A1, A2, B, 01, 01V, and 02) of the ABO blood group system, the high specificity and sensitivity make it suitable also in forensic science. It is more rapid than RFLP and SSCP analysis, resulting in unambiguous interpretation of ABO genotypes and newly discovered mutations are readily investigated by the addition of new extension primers in the minisequencing multiplex reaction.

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Year:  2009        PMID: 18839104     DOI: 10.1007/978-1-59745-553-4_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  1 in total

1.  A new strategy to identify rare blood donors: single polymerase chain reaction multiplex SNaPshot reaction for detection of 16 blood group alleles.

Authors:  Flavia Roche Moreira Latini; Diana Gazito; Carine Prisco Arnoni; Janaína Guilhem Muniz; Rosangela de Medeiros Person; Fabricio Oliveira Carvalho; Wilson Baleotti; Lilian Castilho; José Augusto Barreto
Journal:  Blood Transfus       Date:  2013-04-15       Impact factor: 3.443

  1 in total

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