OBJECTIVE: To examine the effect of deglycosylation on gating properties of rNav1.3. METHODS: rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. RESULTS: Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. CONCLUSION: Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.
OBJECTIVE: To examine the effect of deglycosylation on gating properties of rNav1.3. METHODS:rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. RESULTS: Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. CONCLUSION: Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.