Literature DB >> 18838786

Thin frozen film method for visualization of storage proteins in mature rice grains.

Yuhi Saito1, Nobuaki Nakatsuka, Takanari Shigemitsu, Kunisuke Tanaka, Shigeto Morita, Shigeru Satoh, Takehiro Masumura.   

Abstract

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.

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Year:  2008        PMID： 18838786     DOI： 10.1271/bbb.80337

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  9 in total

1.  Production of human growth hormone in transgenic rice seeds: co-introduction of RNA interference cassette for suppressing the gene expression of endogenous storage proteins.

Authors:  Takanari Shigemitsu; Shinji Ozaki; Yuhi Saito; Masaharu Kuroda; Shigeto Morita; Shigeru Satoh; Takehiro Masumura
Journal:  Plant Cell Rep       Date:  2011-11-23       Impact factor: 4.570

2.  The localization of rice prolamin species in protein body type I is determined by the temporal control of gene expression of the respective prolamin promoters.

Authors:  Ai Sasou; Takanari Shigemitsu; Shigeto Morita; Takehiro Masumura
Journal:  Plant Biotechnol (Tokyo)       Date:  2018-12-25       Impact factor: 1.133

3.  Accumulation of rice prolamin-GFP fusion proteins induces ER-derived protein bodies in transgenic rice calli.

Authors:  Takanari Shigemitsu; Takehiro Masumura; Shigeto Morita; Shigeru Satoh
Journal:  Plant Cell Rep       Date:  2012-11-29       Impact factor: 4.570

4.  Additional nitrogen fertilization at heading time of rice down-regulates cellulose synthesis in seed endosperm.

Authors:  Keiko Midorikawa; Masaharu Kuroda; Kaede Terauchi; Masako Hoshi; Sachiko Ikenaga; Yoshiro Ishimaru; Keiko Abe; Tomiko Asakura
Journal:  PLoS One       Date:  2014-06-06       Impact factor: 3.240

5.  Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.

Authors:  Ai Sasou; Takanari Shigemitsu; Yuhi Saito; Manami Tanaka; Shigeto Morita; Takehiro Masumura
Journal:  Plant Cell Rep       Date:  2016-02-24       Impact factor: 4.570

6.  Accumulation of foreign polypeptides to rice seed protein body type I using prolamin portion sequences.

Authors:  Ai Sasou; Takanari Shigemitsu; Shigeto Morita; Takehiro Masumura
Journal:  Plant Cell Rep       Date:  2016-12-27       Impact factor: 4.570

7.  Proteomic analysis of reserve proteins in commercial rice cultivars.

Authors:  Sara Graziano; Nelson Marmiroli; Mariolina Gullì
Journal:  Food Sci Nutr       Date:  2020-02-25       Impact factor: 2.863

8.  Laser microdissection transcriptome data derived gene regulatory networks of developing rice endosperm revealed tissue- and stage-specific regulators modulating starch metabolism.

Authors:  Tsutomu Ishimaru; Sabiha Parween; Yuhi Saito; Takehiro Masumura; Motohiko Kondo; Nese Sreenivasulu
Journal:  Plant Mol Biol       Date:  2022-01-31       Impact factor: 4.076

9.  A green fluorescent protein fused to rice prolamin forms protein body-like structures in transgenic rice.

Authors:  Yuhi Saito; Koichi Kishida; Kenji Takata; Hideyuki Takahashi; Takeaki Shimada; Kunisuke Tanaka; Shigeto Morita; Shigeru Satoh; Takehiro Masumura
Journal:  J Exp Bot       Date:  2009-01-06       Impact factor: 6.992
  9 in total

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