| Literature DB >> 1883836 |
S Sakajo1, N Minagawa, T Komiyama, A Yoshimoto.
Abstract
A cDNA for mRNA induced by antimycin A in Hansenula anomala was cloned. The mRNA for the cDNA was expressed in the yeast under the conditions expressing the cyanide-resistant respiration activity. The nucleotide sequence revealed a long open reading frame of 342 codons encoding a protein with a molecular weight of 40,282 in the cDNA. An antibody recognizing the protein encoded by the open reading frame was produced. Immunoblotting of H. anomala proteins with this antibody showed that a 36 kDa protein localized in mitochondria was a mature form of the protein encoded by the cDNA. It is suggested that the cloned cDNA encodes a protein involved in the cyanide-resistant respiratory pathway.Entities:
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Year: 1991 PMID： 1883836 DOI： 10.1016/0167-4781(91)90043-l
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002