Literature DB >> 18838128

PKU-beta/TLK1 regulates myosin II activities, and is required for accurate equaled chromosome segregation.

Mitsumasa Hashimoto1, Tadashi Matsui, Kuniyoshi Iwabuchi, Takayasu Date.   

Abstract

Tousled-like kinase 1 (or protein kinase ubiquitous, PKU-beta/TLK1) is a serine/threonine protein kinase that is implicated in chromatin remodeling, DNA replication and mitosis. RNAi-mediated PKU-beta/TLK1-depleted human cells showed aneuploidy, and immunofluorescence analysis of these cells revealed the unequal segregation of daughter chromosomes. Immunoblots indicated a substantial reduction in the phosphorylation level of Ser19/Thr18 on the myosin II regulatory light chain (MRLC) in PKU-beta/TLK1-depleted cells, with no change in total MRLC protein. To confirm the relationship between mitotic aberration and MRLC dysfunction, we expressed wild type MRLC or DD-MRLC (mimics diphosphorylation; substitution of both Thr18 and Ser19 with aspartate) in PKU-beta/TLK1-depleted cells. DD-MRLC expression dramatically reduced the unequal segregation of chromosomes. Our data suggest that human PKU-beta/TLK1 plays an important role in chromosome integrity via the regulation of myosin II dynamics by phosphorylating MRLC during mitosis.

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Year:  2008        PMID： 18838128     DOI： 10.1016/j.mrgentox.2008.09.001

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  10 in total

1.  Identification of the proteome complement of humanTLK1 reveals it binds and phosphorylates NEK1 regulating its activity.

Authors:  Vibha Singh; Zachary M Connelly; Xinggui Shen; Arrigo De Benedetti
Journal:  Cell Cycle       Date:  2017-04-20       Impact factor: 4.534

2.  Tousled-like kinases modulate reactivation of gammaherpesviruses from latency.

Authors:  Patrick J Dillon; Sean M Gregory; Kristen Tamburro; Marcia K Sanders; Gary L Johnson; Nancy Raab-Traub; Dirk P Dittmer; Blossom Damania
Journal:  Cell Host Microbe       Date:  2013-02-13       Impact factor: 21.023

Review 3.  Myosin light chain kinases and phosphatase in mitosis and cytokinesis.

Authors:  Fumio Matsumura; Yoshihiko Yamakita; Shigeko Yamashiro
Journal:  Arch Biochem Biophys       Date:  2011-03-21       Impact factor: 4.013

Review 4.  Mitotic crisis: the unmasking of a novel role for RPA.

Authors:  Rachel William Anantha; James A Borowiec
Journal:  Cell Cycle       Date:  2009-02-21       Impact factor: 4.534

5.  Recombinant AAV9-TLK1B administration ameliorates fractionated radiation-induced xerostomia.

Authors:  Prakash Srinivasan Timiri Shanmugam; Robert D Dayton; Senthilnathan Palaniyandi; Fleurette Abreo; Gloria Caldito; Ronald L Klein; Gulshan Sunavala-Dossabhoy
Journal:  Hum Gene Ther       Date:  2013-06       Impact factor: 5.695

6.  Optimization based tumor classification from microarray gene expression data.

Authors:  Onur Dagliyan; Fadime Uney-Yuksektepe; I Halil Kavakli; Metin Turkay
Journal:  PLoS One       Date:  2011-02-04       Impact factor: 3.240

7.  The Tousled-Like Kinases as Guardians of Genome Integrity.

Authors:  Arrigo De Benedetti
Journal:  ISRN Mol Biol       Date:  2012-01-01

8.  Tousled-like kinases phosphorylate Asf1 to promote histone supply during DNA replication.

Authors:  Ilnaz M Klimovskaia; Clifford Young; Caroline B Strømme; Patrice Menard; Zuzana Jasencakova; Jakob Mejlvang; Katrine Ask; Michael Ploug; Michael L Nielsen; Ole N Jensen; Anja Groth
Journal:  Nat Commun       Date:  2014-03-06       Impact factor: 14.919

9.  Purification, crystallization and preliminary X-ray diffraction analysis of the kinase domain of human tousled-like kinase 2.

Authors:  Ana M Garrote; Pilar Redondo; Guillermo Montoya; Inés G Muñoz
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-02-19       Impact factor: 1.056

10.  De Novo and Inherited Loss-of-Function Variants in TLK2: Clinical and Genotype-Phenotype Evaluation of a Distinct Neurodevelopmental Disorder.

Authors:  Margot R F Reijnders; Kerry A Miller; Mohsan Alvi; Jacqueline A C Goos; Melissa M Lees; Anna de Burca; Alex Henderson; Alison Kraus; Barbara Mikat; Bert B A de Vries; Bertrand Isidor; Bronwyn Kerr; Carlo Marcelis; Caroline Schluth-Bolard; Charu Deshpande; Claudia A L Ruivenkamp; Dagmar Wieczorek; Diana Baralle; Edward M Blair; Hartmut Engels; Hermann-Josef Lüdecke; Jacqueline Eason; Gijs W E Santen; Jill Clayton-Smith; Kate Chandler; Katrina Tatton-Brown; Katelyn Payne; Katherine Helbig; Kelly Radtke; Kimberly M Nugent; Kirsten Cremer; Tim M Strom; Lynne M Bird; Margje Sinnema; Maria Bitner-Glindzicz; Marieke F van Dooren; Marielle Alders; Marije Koopmans; Lauren Brick; Mariya Kozenko; Megan L Harline; Merel Klaassens; Michelle Steinraths; Nicola S Cooper; Patrick Edery; Patrick Yap; Paulien A Terhal; Peter J van der Spek; Phillis Lakeman; Rachel L Taylor; Rebecca O Littlejohn; Rolph Pfundt; Saadet Mercimek-Andrews; Alexander P A Stegmann; Sarina G Kant; Scott McLean; Shelagh Joss; Sigrid M A Swagemakers; Sofia Douzgou; Steven A Wall; Sébastien Küry; Eduardo Calpena; Nils Koelling; Simon J McGowan; Stephen R F Twigg; Irene M J Mathijssen; Christoffer Nellaker; Han G Brunner; Andrew O M Wilkie
Journal:  Am J Hum Genet       Date:  2018-05-31       Impact factor: 11.025
  10 in total

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