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Literature DB >> 18836021

The Functional quality of soluble recombinant polypeptides produced in Escherichia coli is defined by a wide conformational spectrum.

Mónica Martínez-Alonso¹, Nuria González-Montalbán, Elena García-Fruitós, Antonio Villaverde.

Abstract

We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.

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Year: 2008 PMID： 18836021 PMCID： PMC2592911 DOI： 10.1128/AEM.01446-08

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

19 in total

1. The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures.

Authors: Andrea Vera; Nuria González-Montalbán; Anna Arís; Antonio Villaverde
Journal: Biotechnol Bioeng Date: 2007-04-15 Impact factor: 4.530

2. Localization of functional polypeptides in bacterial inclusion bodies.

Authors: Elena García-Fruitós; Anna Arís; Antonio Villaverde
Journal: Appl Environ Microbiol Date: 2006-11-03 Impact factor: 4.792

3. Physiological aggregation of maltodextrin phosphorylase from Pyrococcus furiosus and its application in a process of batch starch degradation to alpha-D-glucose-1-phosphate.

Authors: Jozef Nahálka
Journal: J Ind Microbiol Biotechnol Date: 2007-12-18 Impact factor: 3.346

4. Recombinant protein solubility - does more mean better?

Authors: Nuria González-Montalbán; Elena García-Fruitós; Antonio Villaverde
Journal: Nat Biotechnol Date: 2007-07 Impact factor: 54.908

5. A crosslinked inclusion body process for sialic acid synthesis.

Authors: Jozef Nahálka; Alica Vikartovská; Eva Hrabárová
Journal: J Biotechnol Date: 2008-03-20 Impact factor: 3.307

6. Divergent genetic control of protein solubility and conformational quality in Escherichia coli.

Authors: Elena García-Fruitós; Mónica Martínez-Alonso; Nuria Gonzàlez-Montalbán; Minoska Valli; Diethard Mattanovich; Antonio Villaverde
Journal: J Mol Biol Date: 2007-09-08 Impact factor: 5.469

7. Properties of soluble fusions between mammalian aspartic proteinases and bacterial maltose-binding protein.

Authors: D Sachdev; J M Chirgwin
Journal: J Protein Chem Date: 1999-01

8. Encapsulation of Trigonopsis variabilis D-amino acid oxidase and fast comparison of the operational stabilities of free and immobilized preparations of the enzyme.

Authors: Jozef Nahalka; Iskandar Dib; Bernd Nidetzky
Journal: Biotechnol Bioeng Date: 2008-02-01 Impact factor: 4.530

9. Characterization of the aggregates formed during recombinant protein expression in bacteria.

Authors: Andrea Schrödel; Ario de Marco
Journal: BMC Biochem Date: 2005-05-31 Impact factor: 4.059

10. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli.

Authors: Hans Peter Sørensen; Kim Kusk Mortensen
Journal: Microb Cell Fact Date: 2005-01-04 Impact factor: 5.328

13 in total

1. Rehosting of bacterial chaperones for high-quality protein production.

Authors: Mónica Martínez-Alonso; Verónica Toledo-Rubio; Rob Noad; Ugutz Unzueta; Neus Ferrer-Miralles; Polly Roy; Antonio Villaverde
Journal: Appl Environ Microbiol Date: 2009-10-09 Impact factor: 4.792

2. Cross-system excision of chaperone-mediated proteolysis in chaperone-assisted recombinant protein production.

Authors: Mónica Martínez-Alonso; Antonio Villaverde; Neus Ferrer-Miralles
Journal: Bioeng Bugs Date: 2009-12-29

3. Engineering building blocks for self-assembling protein nanoparticles.

Authors: Esther Vázquez; Antonio Villaverde
Journal: Microb Cell Fact Date: 2010-12-30 Impact factor: 5.328

4. Recombinant Protein Production and Purification of Insoluble Proteins.

Authors: Neus Ferrer-Miralles; Paolo Saccardo; José Luis Corchero; Elena Garcia-Fruitós
Journal: Methods Mol Biol Date: 2022

5. Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm.

Authors: Riccardo Villa; Marina Lotti; Pietro Gatti-Lafranconi
Journal: Microb Cell Fact Date: 2009-06-05 Impact factor: 5.328

Review 6. Side effects of chaperone gene co-expression in recombinant protein production.

Authors: Mónica Martínez-Alonso; Elena García-Fruitós; Neus Ferrer-Miralles; Ursula Rinas; Antonio Villaverde
Journal: Microb Cell Fact Date: 2010-09-02 Impact factor: 5.328

7. Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli.

Authors: Hugo G Menzella
Journal: Microb Cell Fact Date: 2011-03-03 Impact factor: 5.328

8. Recombinant protein quality evaluation: proposal for a minimal information standard.

Authors: Ashley M Buckle; Mark A Bate; Steve Androulakis; Mario Cinquanta; Jerome Basquin; Fabien Bonneau; Deb K Chatterjee; Davide Cittaro; Susanne Gräslund; Alicja Gruszka; Rebecca Page; Sabine Suppmann; Jun X Wheeler; Deborah Agostini; Mike Taussig; Chris F Taylor; Stephen P Bottomley; Antonio Villaverde; Ario de Marco
Journal: Stand Genomic Sci Date: 2011-11-22

9. Intracellular CXCR4⁺ cell targeting with T22-empowered protein-only nanoparticles.

Authors: Ugutz Unzueta; María Virtudes Céspedes; Neus Ferrer-Miralles; Isolda Casanova; Juan Cedano; José Luis Corchero; Joan Domingo-Espín; Antonio Villaverde; Ramón Mangues; Esther Vázquez
Journal: Int J Nanomedicine Date: 2012-08-15

10. Learning about protein solubility from bacterial inclusion bodies.

Authors: Mónica Martínez-Alonso; Nuria González-Montalbán; Elena García-Fruitós; Antonio Villaverde
Journal: Microb Cell Fact Date: 2009-01-08 Impact factor: 5.328