Characterization of ADAR1-mediated modulation of gene expression.

Conversion of adenosine into inosine in RNA molecules constitutes an important post-transcriptional mechanism for generating transcript diversity and is catalyzed by adenosine deaminases acting on RNA (ADARs). Intriguingly, we observed that the editing enzyme ADAR1 enhances reporter gene expression in a cellular, plasmid-based system. The induction of gene expression is independent of the used reporter transgene or the promoter type, but relies on the RNA editing activity and specificity of ADAR1. More detailed analysis indicates that the effect is due to enhanced reporter gene transcription. Induction of gene expression by ADAR1 is lost when the reporter expression cassette is placed in a chromosomal environment. Our results suggest that a cellular, ADAR1-specific RNA editing substrate causes upregulation of plasmid-based gene expression.