OBJECTIVE: This study aimed to identify the oncogenes at 20q involved in colorectal adenoma to carcinoma progression by measuring the effect of 20q gain on mRNA expression of genes in this amplicon. METHODS: Segmentation of DNA copy number changes on 20q was performed by array CGH (comparative genomic hybridisation) in 34 non-progressed colorectal adenomas, 41 progressed adenomas (ie, adenomas that present a focus of cancer) and 33 adenocarcinomas. Moreover, a robust analysis of altered expression of genes in these segments was performed by microarray analysis in 37 adenomas and 31 adenocarcinomas. Protein expression was evaluated by immunohistochemistry on tissue microarrays. RESULTS: The genes C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5, mapping at 20q, were significantly overexpressed in carcinomas compared with adenomas as a consequence of copy number gain of 20q. CONCLUSION: This approach revealed C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5 genes to be important in chromosomal instability-related adenoma to carcinoma progression. These genes therefore may serve as highly specific biomarkers for colorectal cancer with potential clinical applications.
OBJECTIVE: This study aimed to identify the oncogenes at 20q involved in colorectal adenoma to carcinoma progression by measuring the effect of 20q gain on mRNA expression of genes in this amplicon. METHODS: Segmentation of DNA copy number changes on 20q was performed by array CGH (comparative genomic hybridisation) in 34 non-progressed colorectal adenomas, 41 progressed adenomas (ie, adenomas that present a focus of cancer) and 33 adenocarcinomas. Moreover, a robust analysis of altered expression of genes in these segments was performed by microarray analysis in 37 adenomas and 31 adenocarcinomas. Protein expression was evaluated by immunohistochemistry on tissue microarrays. RESULTS: The genes C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5, mapping at 20q, were significantly overexpressed in carcinomas compared with adenomas as a consequence of copy number gain of 20q. CONCLUSION: This approach revealed C20orf24, AURKA, RNPC1, TH1L, ADRM1, C20orf20 and TCFL5 genes to be important in chromosomal instability-related adenoma to carcinoma progression. These genes therefore may serve as highly specific biomarkers for colorectal cancer with potential clinical applications.
Authors: Jochen Gaedcke; Marian Grade; Klaus Jung; Jordi Camps; Peter Jo; Georg Emons; Anastasia Gehoff; Ulrich Sax; Markus Schirmer; Heinz Becker; Tim Beissbarth; Thomas Ried; B Michael Ghadimi Journal: Genes Chromosomes Cancer Date: 2010-11 Impact factor: 5.006
Authors: Sean D Ryan; Eric M C Britigan; Lauren M Zasadil; Kristen Witte; Anjon Audhya; Avtar Roopra; Beth A Weaver Journal: Proc Natl Acad Sci U S A Date: 2012-07-09 Impact factor: 11.205
Authors: Jakob Albrethsen; Jaco C Knol; Sander R Piersma; Thang V Pham; Meike de Wit; Sandra Mongera; Beatriz Carvalho; Henk M W Verheul; Remond J A Fijneman; Gerrit A Meijer; Connie R Jimenez Journal: Mol Cell Proteomics Date: 2010-01-20 Impact factor: 5.911
Authors: Kristina K Lagerstedt; Erik Kristiansson; Christina Lönnroth; Marianne Andersson; Britt-Marie Iresjö; Annika Gustafsson; Elisabeth Hansson; Ulf Kressner; Svante Nordgren; Fredrik Enlund; Kent Lundholm Journal: Cancer Inform Date: 2010-04-23
Authors: Anke H Sillars-Hardebol; Beatriz Carvalho; Meike de Wit; Cindy Postma; Pien M Delis-van Diemen; Sandra Mongera; Bauke Ylstra; Mark A van de Wiel; Gerrit A Meijer; Remond J A Fijneman Journal: Tumour Biol Date: 2010-01-21
Authors: José L Costa; Paul P Eijk; Mark A van de Wiel; Derk ten Berge; Fernando Schmitt; Carmen J Narvaez; JoEllen Welsh; Bauke Ylstra Journal: BMC Genomics Date: 2009-10-28 Impact factor: 3.969