| Literature DB >> 18828843 |
U Toprak1, D Baldwin, M Erlandson, C Gillott, X Hou, C Coutu, D D Hegedus.
Abstract
One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.Entities:
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Year: 2008 PMID： 18828843 DOI： 10.1111/j.1365-2583.2008.00829.x
Source DB: PubMed Journal: Insect Mol Biol ISSN： 0962-1075 Impact factor: 3.585