Literature DB >> 1882705

A luminometric method for the determination of ATP and phosphocreatine in single human skeletal muscle fibres.

R Wibom1, K Söderlund, A Lundin, E Hultman.   

Abstract

A sensitive method for the analysis of ATP and phosphocreatine (PCr) in single human skeletal muscle fibres is described. Muscle tissue was freeze-dried and single fibres were dissected free with the aid of low-power microscopy. The fibres were then extracted in trichloroacetic acid and neutralized with KHCO3. The assay is based on the continuous monitoring of light produced as a result of ATP degradation in the firefly luciferase reaction. PCr is measured as the amount of ATP formed in the creatine kinase reaction. The coefficient of variation was less than 4% for both ATP and PCr determination. The amount of tissue required for the assay is approximately 0.5 microgram (dry weight). The assay showed good agreement with spectrophotometric and high-performance liquid chromatographic (HPLC) measurements made upon extracts of whole muscle tissue.

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Year:  1991        PMID： 1882705     DOI： 10.1002/bio.1170060210

Source DB:  PubMed          Journal:  J Biolumin Chemilumin        ISSN： 0884-3996


  9 in total

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