BACKGROUND & AIMS: In this study, the inhibitory effect of olive oil on liver fibrosis induced by carbon tetrachloride (CCl(4)) has been investigated in rats. METHODS: Rats were divided randomly into four groups: control, CCl(4), and two olive oil groups. Except for rats in the control group, all rats were orally administered CCl(4) twice a week for 8 weeks. Rats in the olive oil groups were treated daily with olive oil (2 or 10 ml/kg) through gastrogavage for the entire experimental period. RESULTS: RT-PCR analysis showed that CCl(4) increased the hepatic mRNA expressions of lipopolysaccharide binding protein, CD14, Toll-like receptor-4, NADPH oxidase, nuclear factor-kappa beta, collagen (alpha1) (I), collagen (alpha1) (III), and transforming growth factor beta1. The expression of these mRNAs could be decreased by olive oil treatment. In addition, Western blot analysis also supported these results. CCl(4)-induced liver damage, as characterized by the increase in hepatic malondialdehyde and hydroxyproline levels. Olive oil treatment decreased the hepatic malondialdehyde and hydroxyproline levels. Histological evaluations showed that olive oil could attenuate the liver fibrosis, necrosis, and expression of smooth muscle alpha-actin that are induced by CCl(4). CONCLUSION: It is speculated that the phenolic compounds in olive oil significantly reduced CCl(4)-induced hepatic fibrosis in rats.
BACKGROUND & AIMS: In this study, the inhibitory effect of olive oil on liver fibrosis induced by carbon tetrachloride (CCl(4)) has been investigated in rats. METHODS:Rats were divided randomly into four groups: control, CCl(4), and two olive oil groups. Except for rats in the control group, all rats were orally administered CCl(4) twice a week for 8 weeks. Rats in the olive oil groups were treated daily with olive oil (2 or 10 ml/kg) through gastrogavage for the entire experimental period. RESULTS: RT-PCR analysis showed that CCl(4) increased the hepatic mRNA expressions of lipopolysaccharide binding protein, CD14, Toll-like receptor-4, NADPH oxidase, nuclear factor-kappa beta, collagen (alpha1) (I), collagen (alpha1) (III), and transforming growth factor beta1. The expression of these mRNAs could be decreased by olive oil treatment. In addition, Western blot analysis also supported these results. CCl(4)-induced liver damage, as characterized by the increase in hepatic malondialdehyde and hydroxyproline levels. Olive oil treatment decreased the hepatic malondialdehyde and hydroxyproline levels. Histological evaluations showed that olive oil could attenuate the liver fibrosis, necrosis, and expression of smooth muscle alpha-actin that are induced by CCl(4). CONCLUSION: It is speculated that the phenolic compounds in olive oil significantly reduced CCl(4)-induced hepatic fibrosis in rats.
Authors: Keren Borensztajn; Paul Bresser; Chris van der Loos; Ilze Bot; Bernt van den Blink; Michael A den Bakker; Joost Daalhuisen; Angelique P Groot; Maikel P Peppelenbosch; Jan H von der Thüsen; C Arnold Spek Journal: Am J Pathol Date: 2010-10-22 Impact factor: 4.307
Authors: Madeha N Al-Seeni; Haddad A El Rabey; Mazin A Zamzami; Abeer M Alnefayee Journal: BMC Complement Altern Med Date: 2016-11-04 Impact factor: 3.659