BACKGROUND: We previously identified a novel exogenous gammaretrovirus (xenotropic murine leukemia virus-related gammaretrovirus (XMRV)) using a pan-viral microarray. XMRV is the first MLV-related virus found in human infection. Forty percent (8/20) of familial prostate cancer patients homozygous for a mutation in RNase L (R462Q) were positive for XMRV, while the virus was rarely (1/66) detected in familial prostate cancer patients heterozygous for R462Q or carrying the wild type allele. OBJECTIVES: To determine the presence of XMRV in non-familial prostate cancer samples. STUDY DESIGN: RNA from prostate tissue was analyzed for XMRV using nested RT-PCR. In all samples, RNase L (R462Q) genotyping was performed using an allele-specific PCR. RESULTS: XMRV-specific sequences were detected in one of 105 tissue samples from non-familial prostate cancer patients and from one of 70 tissue samples from men without prostate cancer. The two XMRV-positive patients were wild type or heterozygous for the R462Q mutation and thus carried at least one fully functional RNase L allele. CONCLUSIONS: XMRV was rarely detected in non-familial prostate cancer samples from Northern European patients. The homozygous mutation R462Q (QQ) was significantly underrepresented (<6%) in this cohort when compared to other studies (11-17%).
BACKGROUND: We previously identified a novel exogenous gammaretrovirus (xenotropic murine leukemia virus-related gammaretrovirus (XMRV)) using a pan-viral microarray. XMRV is the first MLV-related virus found in humaninfection. Forty percent (8/20) of familial prostate cancerpatients homozygous for a mutation in RNase L (R462Q) were positive for XMRV, while the virus was rarely (1/66) detected in familial prostate cancerpatients heterozygous for R462Q or carrying the wild type allele. OBJECTIVES: To determine the presence of XMRV in non-familial prostate cancer samples. STUDY DESIGN: RNA from prostate tissue was analyzed for XMRV using nested RT-PCR. In all samples, RNase L (R462Q) genotyping was performed using an allele-specific PCR. RESULTS:XMRV-specific sequences were detected in one of 105 tissue samples from non-familial prostate cancerpatients and from one of 70 tissue samples from men without prostate cancer. The two XMRV-positive patients were wild type or heterozygous for the R462Q mutation and thus carried at least one fully functional RNase L allele. CONCLUSIONS:XMRV was rarely detected in non-familial prostate cancer samples from Northern European patients. The homozygous mutation R462Q (QQ) was significantly underrepresented (<6%) in this cohort when compared to other studies (11-17%).
Authors: Romana Hadravová; Alex de Marco; Pavel Ulbrich; Jitka Stokrová; Michal Dolezal; Iva Pichová; Tomás Ruml; John A G Briggs; Michaela Rumlová Journal: J Virol Date: 2011-11-16 Impact factor: 5.103
Authors: Shixing Tang; Jiangqin Zhao; Ragupathy Viswanath; Phillipe N Nyambi; Andrew D Redd; Armeta Dastyar; Lisa A Spacek; Thomas C Quinn; Xue Wang; Owen Wood; Durga Gaddam; Krishnakumar Devadas; Indira K Hewlett Journal: Transfusion Date: 2010-11-15 Impact factor: 3.157
Authors: Otto Erlwein; Steve Kaye; Myra O McClure; Jonathan Weber; Gillian Wills; David Collier; Simon Wessely; Anthony Cleare Journal: PLoS One Date: 2010-01-06 Impact factor: 3.240
Authors: Oliver Hohn; Hans Krause; Pia Barbarotto; Lars Niederstadt; Nadine Beimforde; Joachim Denner; Kurt Miller; Reinhard Kurth; Norbert Bannert Journal: Retrovirology Date: 2009-10-16 Impact factor: 4.602