The use of a nested PCR-RFLP technique, based on the parasite's 18S ribosomal RNA, to characterise Cryptosporidium isolates from HIV/AIDS patients.

Since there have been few studies on human cryptosporidiosis in Iran, attempts were made to identify Cryptosporidium isolates from HIV-positive Iranians, to genotype level. A nested PCR (based on a fragment of the parasite's 18S ribosomal-RNA gene) was first used to see if faecal samples from 35 HIV-positive patients (of whom 17 had apparently been found smear-positive for Cryptosporidium oocysts) contained Cryptosporidium. Twenty-one of the samples (including all 17 of those that appeared smear-positive) were found PCR-positive. Each of these 21 samples was then investigated further, by RFLP analysis in which the amplicons from the secondary PCR were digested with VspI. Curiously, although HIV-infected individuals are known to be susceptible to infection with a wide range of Cryptosporidium genotypes, all the Iranian subjects of the present study were found to be infected with C. hominis (71%) or C. parvum (29%).