BACKGROUND: We have developed a method, termed laser-activated nano-thermolysis as a cell elimination technology (LANTCET), for the selective detection and destruction of individual tumor cells by the generation of intracellular photothermal bubbles around clusters of gold nanoparticles. METHOD: Bare nanoparticles and their conjugates to C225 tumor-specific monoclonal antibodies were applied in vitro to C225-positive squamous carcinoma cells and in vivo to an experimental tumor in a rat in order to form intracellular clusters of nanoparticles. RESULTS: Single 10 ns laser pulses generated intracellular photothermal microbubbles at a near-infrared and visible wavelengths. The cells with the clusters yielded an almost 100-fold decrease in the laser fluence threshold for bubble generation and cell damage relative to that for the cells without clusters. Cell damage had a mechanical origin and single cell selectivity. Three LANTCET processes (cell detection, damage and optical guidance) were realized as a microsecond sequence and with the one device.
BACKGROUND: We have developed a method, termed laser-activated nano-thermolysis as a cell elimination technology (LANTCET), for the selective detection and destruction of individual tumor cells by the generation of intracellular photothermal bubbles around clusters of gold nanoparticles. METHOD: Bare nanoparticles and their conjugates to C225 tumor-specific monoclonal antibodies were applied in vitro to C225-positive squamous carcinoma cells and in vivo to an experimental tumor in a rat in order to form intracellular clusters of nanoparticles. RESULTS: Single 10 ns laser pulses generated intracellular photothermal microbubbles at a near-infrared and visible wavelengths. The cells with the clusters yielded an almost 100-fold decrease in the laser fluence threshold for bubble generation and cell damage relative to that for the cells without clusters. Cell damage had a mechanical origin and single cell selectivity. Three LANTCET processes (cell detection, damage and optical guidance) were realized as a microsecond sequence and with the one device.
Authors: Ekaterina Y Lukianova-Hleb; Irina I Koneva; Alexander O Oginsky; Saverio La Francesca; Dmitri O Lapotko Journal: J Surg Res Date: 2010-11-26 Impact factor: 2.192
Authors: Ekaterina Y Lukianova-Hleb; Yoo-Shin Kim; Ihor Belatsarkouski; Ann M Gillenwater; Brian E O'Neill; Dmitri O Lapotko Journal: Nat Nanotechnol Date: 2016-02-15 Impact factor: 39.213
Authors: Ekaterina Y Lukianova-Hleb; Alexander O Oginsky; Derek L Shenefelt; Rebekah A Drezek; Jason H Hafner; Mary C Farach-Carson; Dmitri O Lapotko Journal: J Nanomed Nanotechnol Date: 2011-01-01
Authors: Ekaterina Y Lukianova-Hleb; Adam P Samaniego; Jianguo Wen; Leonid S Metelitsa; Chung-Che Chang; Dmitri O Lapotko Journal: J Control Release Date: 2011-02-17 Impact factor: 9.776