OBJECTIVE: Electroporation has been proved to enhance the efficacy of intramuscular delivery of DNA. However, the process of electroporation causes pain and discomfort to the patient receiving the treatment. Higher the electroporation voltages inflict greater pain, and this limits the circumstances in which the technique can be used clinically. The voltages generally used for electroporation in animals range from 100 to 1,200 V/cm. We studied the effect of DNA vaccination when electroporation was performed at lower voltages. METHODS: BALB/c mice were immunized twice by electroporation, at a 3-week interval, with H5N1 virus hemagglutinin (HA) DNA. One week after the booster had been given, the mice were challenged with a lethal dose of mouse-adapted H5N1 virus. The immune effects of HA DNA were evaluated by the survival rate, lung virus titer, bodyweight change and antibody titer of the mice. RESULTS: The higher the voltage used, the more able were the mice to survive the challenge. However, a significant degree of protection could also be achieved with a voltage as low as 5 V. When electroporation was performed at a voltage of 10 V, an immunization amount of 5 microg is enough for HA DNA to provide effective protection. CONCLUSION: Low-voltage electroporation can induce immunity and protect mice effectively. There is, therefore, the potential to reduce the voltages currently used for DNA electroporation. Copyright 2008 S. Karger AG, Basel.
OBJECTIVE: Electroporation has been proved to enhance the efficacy of intramuscular delivery of DNA. However, the process of electroporation causes pain and discomfort to the patient receiving the treatment. Higher the electroporation voltages inflict greater pain, and this limits the circumstances in which the technique can be used clinically. The voltages generally used for electroporation in animals range from 100 to 1,200 V/cm. We studied the effect of DNA vaccination when electroporation was performed at lower voltages. METHODS: BALB/c mice were immunized twice by electroporation, at a 3-week interval, with H5N1 virus hemagglutinin (HA) DNA. One week after the booster had been given, the mice were challenged with a lethal dose of mouse-adapted H5N1 virus. The immune effects of HA DNA were evaluated by the survival rate, lung virus titer, bodyweight change and antibody titer of the mice. RESULTS: The higher the voltage used, the more able were the mice to survive the challenge. However, a significant degree of protection could also be achieved with a voltage as low as 5 V. When electroporation was performed at a voltage of 10 V, an immunization amount of 5 microg is enough for HA DNA to provide effective protection. CONCLUSION: Low-voltage electroporation can induce immunity and protect mice effectively. There is, therefore, the potential to reduce the voltages currently used for DNA electroporation. Copyright 2008 S. Karger AG, Basel.
Authors: Thomas Niezold; Michael Storcksdieck Genannt Bonsmann; André Maaske; Vladimir Temchura; Vanessa Heinecke; Drew Hannaman; Jan Buer; Christina Ehrhardt; Wiebke Hansen; Klaus Überla; Matthias Tenbusch Journal: Immunology Date: 2015-04-30 Impact factor: 7.397
Authors: Viktoria Stab; Sandra Nitsche; Thomas Niezold; Michael Storcksdieck Genannt Bonsmann; Andrea Wiechers; Bettina Tippler; Drew Hannaman; Christina Ehrhardt; Klaus Uberla; Thomas Grunwald; Matthias Tenbusch Journal: PLoS One Date: 2013-08-14 Impact factor: 3.240