Richard Bradbury1, Alan Champion, David W Reid. 1. Respiratory Research Group, Menzies Research Institute, University of Tasmania, Collins Street, Hobart, Tasmania, Australia. rbradbur@utas.edu.au
Abstract
BACKGROUND AND OBJECTIVE: Clonal strains of Pseudomonas aeruginosa have been identified in large cystic fibrosis (CF) centres. Whether such strains are more virulent or whether cross-infection between patients explains their widespread prevalence is unknown. This study described the epidemiology of P. aeruginosa infection in CF patients in Tasmania, Australia, an area with a high CF birth incidence. Patients in Tasmania are geographically dispersed and when this study was conducted (2003) there was no central CF clinic, with patients receiving treatment in regional hospitals. METHODS: P. aeruginosa isolates from CF adults aged 15 years and over in Tasmania were genotyped using random amplified polymorphic DNA (RAPD)-PCR and clonal strains confirmed with pulsed field gel electrophoresis. RESULTS: Airway samples were obtained from 41 patients (82% of the adult CF population). P. aeruginosa was isolated from 34 patients and nine (26%) of these individuals harboured P. aeruginosa strains with identical RAPD-PCR and pulsed field gel electrophoresis patterns (Australian Epidemic Strain III--AES III). AES III was isolated from patients in all regions of Tasmania and was distinct from the epidemic CF strains described on mainland Australia (AES I and II). The possible link between CF adults infected with AES III was attendance at family camps more than 12 years previously. Patients harbouring AES III had suffered significantly more exacerbations requiring hospitalisation during the 2 years prior to the study compared with patients infected with unique strains (P < 0.01). AES III displayed increased multi-antibiotic resistance compared with other strains (P < 0.001). CONCLUSIONS: Clonal strains of P. aeruginosa may arise even in isolated CF populations. The increased exacerbation rate in patients infected with AES III and its antibiotic resistance profile strongly suggest increased virulence.
BACKGROUND AND OBJECTIVE: Clonal strains of Pseudomonas aeruginosa have been identified in large cystic fibrosis (CF) centres. Whether such strains are more virulent or whether cross-infection between patients explains their widespread prevalence is unknown. This study described the epidemiology of P. aeruginosa infection in CF patients in Tasmania, Australia, an area with a high CF birth incidence. Patients in Tasmania are geographically dispersed and when this study was conducted (2003) there was no central CF clinic, with patients receiving treatment in regional hospitals. METHODS:P. aeruginosa isolates from CF adults aged 15 years and over in Tasmania were genotyped using random amplified polymorphic DNA (RAPD)-PCR and clonal strains confirmed with pulsed field gel electrophoresis. RESULTS: Airway samples were obtained from 41 patients (82% of the adult CF population). P. aeruginosa was isolated from 34 patients and nine (26%) of these individuals harboured P. aeruginosa strains with identical RAPD-PCR and pulsed field gel electrophoresis patterns (Australian Epidemic Strain III--AES III). AES III was isolated from patients in all regions of Tasmania and was distinct from the epidemic CF strains described on mainland Australia (AES I and II). The possible link between CF adults infected with AES III was attendance at family camps more than 12 years previously. Patients harbouring AES III had suffered significantly more exacerbations requiring hospitalisation during the 2 years prior to the study compared with patients infected with unique strains (P < 0.01). AES III displayed increased multi-antibiotic resistance compared with other strains (P < 0.001). CONCLUSIONS: Clonal strains of P. aeruginosa may arise even in isolated CF populations. The increased exacerbation rate in patients infected with AES III and its antibiotic resistance profile strongly suggest increased virulence.
Authors: Michael D Parkins; Bryan A Glezerson; Christopher D Sibley; Kristen A Sibley; Jessica Duong; Swathi Purighalla; Christopher H Mody; Matthew L Workentine; Douglas G Storey; Michael G Surette; Harvey R Rabin Journal: J Clin Microbiol Date: 2014-01-22 Impact factor: 5.948
Authors: Anthony De Soyza; Amanda J Hall; Eshwar Mahenthiralingam; Pavel Drevinek; Wieslaw Kaca; Zuzanna Drulis-Kawa; Stoyanka R Stoitsova; Veronika Toth; Tom Coenye; James E A Zlosnik; Jane L Burns; Isabel Sá-Correia; Daniel De Vos; Jean-Paul Pirnay; Timothy J Kidd; David Reid; Jim Manos; Jens Klockgether; Lutz Wiehlmann; Burkhard Tümmler; Siobhán McClean; Craig Winstanley Journal: Microbiologyopen Date: 2013-11-11 Impact factor: 3.139
Authors: V Waters; J E A Zlosnik; Y C W Yau; D P Speert; S D Aaron; D S Guttman Journal: Eur J Clin Microbiol Infect Dis Date: 2012-07-29 Impact factor: 3.267
Authors: Nathan J Hare; Nestor Solis; Christopher Harmer; N Bishara Marzook; Barbara Rose; Colin Harbour; Ben Crossett; Jim Manos; Stuart J Cordwell Journal: BMC Microbiol Date: 2012-01-22 Impact factor: 3.605
Authors: Erin M Anderson; Neethu Shaji Saji; Alexander C Anderson; Dyanne Brewer; Anthony J Clarke; Cezar M Khursigara Journal: mSystems Date: 2022-05-12 Impact factor: 7.324
Authors: Jean-Paul Pirnay; Florence Bilocq; Bruno Pot; Pierre Cornelis; Martin Zizi; Johan Van Eldere; Pieter Deschaght; Mario Vaneechoutte; Serge Jennes; Tyrone Pitt; Daniel De Vos Journal: PLoS One Date: 2009-11-13 Impact factor: 3.240