AIM: To study the relationship between nm23H1 gene genetic instability and its clinical pathological characteristics in Chinese digestive system cancer patients. METHODS: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the microsatellite instability (MSI) and loss of heterozygosity (LOH). Immunohistochemistry was employed to detect the expression of nm23H1. RESULTS: The MSI was higher in TNM stage I + II than in stage III + IV of gastric, colonic and gallbladder carcinomas. The LOH was higher in TNM stage III + IV than in stage I + II of gastric, colonic and hepatocellular carcinomas. Lymphatic metastasis was also observed. The expression of nm23H1 protein was lower in TNM stage III + IV than in stage I + II of these tumors and in patients with lymphatic metastasis.The nm23H1 protein expression was higher in the LOH negative group than in the LOH positive group. CONCLUSION: MSI and LOH may independently control the biological behaviors of digestive system cancers. MSI could serve as an early biological marker of digestive system cancers. Enhanced expression of nm23H1 protein could efficiently inhibit cancer metastasis and improve its prognosis. LOH mostly appears in late digestive system cancer.
AIM: To study the relationship between nm23H1 gene genetic instability and its clinical pathological characteristics in Chinese digestive system cancerpatients. METHODS: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the microsatellite instability (MSI) and loss of heterozygosity (LOH). Immunohistochemistry was employed to detect the expression of nm23H1. RESULTS: The MSI was higher in TNM stage I + II than in stage III + IV of gastric, colonic and gallbladder carcinomas. The LOH was higher in TNM stage III + IV than in stage I + II of gastric, colonic and hepatocellular carcinomas. Lymphatic metastasis was also observed. The expression of nm23H1 protein was lower in TNM stage III + IV than in stage I + II of these tumors and in patients with lymphatic metastasis.The nm23H1 protein expression was higher in the LOH negative group than in the LOH positive group. CONCLUSION: MSI and LOH may independently control the biological behaviors of digestive system cancers. MSI could serve as an early biological marker of digestive system cancers. Enhanced expression of nm23H1 protein could efficiently inhibit cancer metastasis and improve its prognosis. LOH mostly appears in late digestive system cancer.
Authors: Maria Nowacka-Zawisza; Magdalena Bryś; Romanowicz-Makowska Hanna; Marek Zadrozny; Andrzej Kulig; Wanda M Krajewska Journal: Pol J Pathol Date: 2006 Impact factor: 1.072
Authors: Monika Korabiowska; Johannes F Hönig; Jacek Jawien; Jadwiga Knapik; Jerzy Stachura; Carlos Cordon-Cardo; Gösta Fischer Journal: In Vivo Date: 2005 Nov-Dec Impact factor: 2.155
Authors: Maximilian Burger; Stefan Denzinger; Christine G Hammerschmied; Andrea Tannapfel; Ellen C Obermann; Wolf F Wieland; Arndt Hartmann; Robert Stoehr Journal: J Mol Med (Berl) Date: 2006-08-03 Impact factor: 4.599
Authors: S Kapitanović; T Cacev; M Berković; M Popović-Hadzija; S Radosević; S Seiwerth; S Spaventi; K Pavelić; R Spaventi Journal: J Clin Pathol Date: 2004-12 Impact factor: 3.411
Authors: Michael G House; James G Herman; Ming Zhou Guo; Craig M Hooker; Richard D Schulick; John L Cameron; Ralph H Hruban; Anirban Maitra; Charles J Yeo Journal: Surgery Date: 2003-12 Impact factor: 3.982