Hao Wu1, Wei Li, Tongshan Wang, Yongqian Shu, Ping Liu. 1. Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, PR China.
Abstract
OBJECTIVE: We sought to determine whether paeoniflorin enhances 5-fluorouracil-induced apoptosis in human gastric carcinoma cells, and, if so, to determine the relationship between this apoptosis and NF-kappaB activation. METHODS: Paeoniflorin was diluted to different concentrations and added to gastric carcinoma cells (SGC-7901) at different times. Western blot was used to test the expression of NF-kappaB in nuclear and I kappaB alpha, p-I kappaB alpha, IKK alpha in cytoplasm. Further, the intranuclear expression of NF-kappaB was confirmed by ELISA assay. The impact of paeoniflorin and 5-fluorouracil on cell apoptosis of gastric carcinoma cells was estimated by flow cytometry. RESULTS: Paeoniflorin revealed dramatic inhibition of NF-kappaB activity in the nuclei of the cells. The inhibition pattern of NF-kappaB was exhibited in a time- and dose-dependent manner, which was confirmed by Western blot and ELISA. Decreased nuclear translocation of NF-kappaB induced by paeoniflorin was found by preventing I kappaB alpha phosphorylation. Moreover, 5-fluorouracil-induced cell apoptosis was promoted by paeoniflorin in gastric carcinoma cells. CONCLUSIONS: Paeoniflorin can inhibit NF-kappaB activity of SGC-7901 cells, and enhance 5-fluorouracil-induced apoptosis of gastric carcinoma cells.
OBJECTIVE: We sought to determine whether paeoniflorin enhances 5-fluorouracil-induced apoptosis in humangastric carcinoma cells, and, if so, to determine the relationship between this apoptosis and NF-kappaB activation. METHODS:Paeoniflorin was diluted to different concentrations and added to gastric carcinoma cells (SGC-7901) at different times. Western blot was used to test the expression of NF-kappaB in nuclear and I kappaB alpha, p-I kappaB alpha, IKK alpha in cytoplasm. Further, the intranuclear expression of NF-kappaB was confirmed by ELISA assay. The impact of paeoniflorin and 5-fluorouracil on cell apoptosis of gastric carcinoma cells was estimated by flow cytometry. RESULTS:Paeoniflorin revealed dramatic inhibition of NF-kappaB activity in the nuclei of the cells. The inhibition pattern of NF-kappaB was exhibited in a time- and dose-dependent manner, which was confirmed by Western blot and ELISA. Decreased nuclear translocation of NF-kappaB induced by paeoniflorin was found by preventing I kappaB alpha phosphorylation. Moreover, 5-fluorouracil-induced cell apoptosis was promoted by paeoniflorin in gastric carcinoma cells. CONCLUSIONS:Paeoniflorin can inhibit NF-kappaB activity of SGC-7901 cells, and enhance 5-fluorouracil-induced apoptosis of gastric carcinoma cells.