R Schmidmaier1, N Oversohl, B Schnabel, C Straka, B Emmerich. 1. Department of Hematology and Oncology, Medizinische Klinik Innenstadt, Klinikum der Universitat Munchen, Munich, Germany. ralf.schmidmaier@med.uni-muenchen.de
Abstract
BACKGROUND: The microenvironment in the bone marrow - including lymphocytes - is part of the pathophysiology of multiple myeloma (MM). High dose chemotherapy followed by autologous stem cell transplantation is standard of care for younger patients. AIM: To determine the influence of reinfused lymphocyte subsets on event free survival (EFS) and overall survival (OS). METHODS: In peripheral blood (PB) and aphaeresis products (AP) of 41 MM patients lymphocyte subsets were determined by flow cytometry and were correlated with clinical outcome. RESULTS: PB lymphocyte subsets did not influence EFS or OS. Residual plasma cells in the AP were not correlated with poor outcome, whereas a high percentage of B cells (CD19+) showed a trend towards reduced EFS (P = 0.051). A high amount of CD4 cells and an increased CD4/CD8 ratio were significantly associated with prolonged EFS. In contrast, high percentage of HLA-DR positive lymphocytes showed negative impact on EFS and OS (P = 0.03 and 0.02, respectively). CONCLUSION: Obtained data suggest the non-activated (HLA-DR negative) helper CD4+ T cells in the AP to be tumour protective.
BACKGROUND: The microenvironment in the bone marrow - including lymphocytes - is part of the pathophysiology of multiple myeloma (MM). High dose chemotherapy followed by autologous stem cell transplantation is standard of care for younger patients. AIM: To determine the influence of reinfused lymphocyte subsets on event free survival (EFS) and overall survival (OS). METHODS: In peripheral blood (PB) and aphaeresis products (AP) of 41 MM patients lymphocyte subsets were determined by flow cytometry and were correlated with clinical outcome. RESULTS: PB lymphocyte subsets did not influence EFS or OS. Residual plasma cells in the AP were not correlated with poor outcome, whereas a high percentage of B cells (CD19+) showed a trend towards reduced EFS (P = 0.051). A high amount of CD4 cells and an increased CD4/CD8 ratio were significantly associated with prolonged EFS. In contrast, high percentage of HLA-DR positive lymphocytes showed negative impact on EFS and OS (P = 0.03 and 0.02, respectively). CONCLUSION: Obtained data suggest the non-activated (HLA-DR negative) helper CD4+ T cells in the AP to be tumour protective.
Authors: Matthew J Rees; Peter Mollee; Jun Yen Ng; Alex Murton; Jose Filipe Gonsalves; Ashish Panigrahi; Hayley Beer; Joanna Loh; Philip Nguyen; Sam Hunt; Hayden Jina; Rebecca Wayte; Gaurav Sutrave; Jocelyn Tan; Chathuri Abeyakoon; Ashlyn Chee; Bradley Augustson; Akash Kalro; Cindy Lee; Shivam Agrawal; Leonid Churilov; Chong Chyn Chua; Andrew Boon Ming Lim; Daniela Zantomio; Andrew Grigg Journal: Bone Marrow Transplant Date: 2021-04-28 Impact factor: 5.483
Authors: Matthew J Rees; Ashish Panigrahi; Simon J Harrison; Andrew Spencer; Tiffany Khong; Simon Gibbs; Jay Hocking; Andrew Grigg; Daniela Zantomio Journal: Bone Marrow Transplant Date: 2022-03-28 Impact factor: 5.174