BACKGROUND: Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infected women will ever develop cervical cancer. Several studies suggested that immunological components play a key role in the development of cervical cancer. Interferon gamma (IFN-gamma ) is a cytokine produced by activated T cells and natural killer (NK) cells that enhances cellular immune responses by increasing T-cell cytotoxicity and NK cell activity. AIM: To study single nucleotide polymorphism (SNP), T to A, located at the +874 position and measure IFN-gamma messenger RNA (mRNA) at the tumor site. METHODS: DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. The allele polymorphism at position +874 in the IFN-gamma gene was studied by ARMS-PCR (Amplification Refractory Mutation System) and measured IFN-gamma mRNA at the tumor site by means of a semi-quantitative polymerase chain reaction (sqRT-PCR) assay. RESULTS: It was observed that genotypes AT and AA + AT increase the risk of cervical cancer (OR = 3.3, 95% CI - 2.05-5.2, P <or= 0.001 - OR = 2.9, 95% CI - 1.9-4.6, P <or= 0.001, respectively). In case of passive smokers same genotypes showed highly significant increased risk of cervical cancer (OR = 5.55, 95% CI = 2.77-11.19 - OR = 5.25, 95% CI = 2.77-10, respectively). Thus, the sqRT-PCR reflected the similar level of mRNA expression of IFN- gamma gene in patients suffering from cervical carcinoma and healthy controls. CONCLUSION: This is the first study to provide an evidence for effecting of IFN-gamma gene on the risk of cervical cancer in north Indian population.
BACKGROUND:Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infectedwomen will ever develop cervical cancer. Several studies suggested that immunological components play a key role in the development of cervical cancer. Interferon gamma (IFN-gamma ) is a cytokine produced by activated T cells and natural killer (NK) cells that enhances cellular immune responses by increasing T-cell cytotoxicity and NK cell activity. AIM: To study single nucleotide polymorphism (SNP), T to A, located at the +874 position and measure IFN-gamma messenger RNA (mRNA) at the tumor site. METHODS: DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. The allele polymorphism at position +874 in the IFN-gamma gene was studied by ARMS-PCR (Amplification Refractory Mutation System) and measured IFN-gamma mRNA at the tumor site by means of a semi-quantitative polymerase chain reaction (sqRT-PCR) assay. RESULTS: It was observed that genotypes AT and AA + AT increase the risk of cervical cancer (OR = 3.3, 95% CI - 2.05-5.2, P <or= 0.001 - OR = 2.9, 95% CI - 1.9-4.6, P <or= 0.001, respectively). In case of passive smokers same genotypes showed highly significant increased risk of cervical cancer (OR = 5.55, 95% CI = 2.77-11.19 - OR = 5.25, 95% CI = 2.77-10, respectively). Thus, the sqRT-PCR reflected the similar level of mRNA expression of IFN- gamma gene in patients suffering from cervical carcinoma and healthy controls. CONCLUSION: This is the first study to provide an evidence for effecting of IFN-gamma gene on the risk of cervical cancer in north Indian population.
Authors: Viviane do Carmo Vasconcelos de Carvalho; Jamilly Lopes de Macêdo; Camilla Albertina Dantas de Lima; Maria da Conceição Gomes de Lima; Sandra de Andrade Heráclio; Melânia Amorim; Maria de Mascena Diniz Maia; Ana Lúcia Figueiredo Porto; Paulo Roberto Eleutério de Souza Journal: Mol Biol Rep Date: 2012-02-12 Impact factor: 2.316