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Literature DB >> 18805998

Effective mutagenesis of Vibrio fischeri by using hyperactive mini-Tn5 derivatives.

Noreen L Lyell¹, Anne K Dunn, Jeffrey L Bose, Susan L Vescovi, Eric V Stabb.

Abstract

We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of approximately 10(-6). This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of V. fischeri and should be applicable to many other bacteria.

Entities: Mutation Species

Mesh：

Substances：

Year: 2008 PMID： 18805998 PMCID： PMC2583470 DOI： 10.1128/AEM.01330-08

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

52 in total

Review 1. An exclusive contract: specificity in the Vibrio fischeri-Euprymna scolopes partnership.

Authors: K L Visick; M J McFall-Ngai
Journal: J Bacteriol Date: 2000-04 Impact factor: 3.490

2. RP4-based plasmids for conjugation between Escherichia coli and members of the Vibrionaceae.

Authors: Eric V Stabb; Edward G Ruby
Journal: Methods Enzymol Date: 2002 Impact factor: 1.600

3. Lateral flagellar gene system of Vibrio parahaemolyticus.

Authors: Bonnie J Stewart; Linda L McCarter
Journal: J Bacteriol Date: 2003-08 Impact factor: 3.490

4. Systematic sequencing of cDNA clones using the transposon Tn5.

Authors: Yuriy Shevchenko; Gerard G Bouffard; Yaron S N Butterfield; Robert W Blakesley; James L Hartley; Alice C Young; Marco A Marra; Steven J M Jones; Jeffrey W Touchman; Eric D Green
Journal: Nucleic Acids Res Date: 2002-06-01 Impact factor: 16.971

5. Alterations in Vibrio fischeri motility correlate with a delay in symbiosis initiation and are associated with additional symbiotic colonization defects.

Authors: Deborah S Millikan; Edward G Ruby
Journal: Appl Environ Microbiol Date: 2002-05 Impact factor: 4.792

6. Chemoattraction of Vibrio fischeri to serine, nucleosides, and N-acetylneuraminic acid, a component of squid light-organ mucus.

Authors: Cindy R DeLoney-Marino; Alan J Wolfe; Karen L Visick
Journal: Appl Environ Microbiol Date: 2003-12 Impact factor: 4.792

7. Isolation of predation-deficient mutants of Bdellovibrio bacteriovorus by using transposon mutagenesis.

Authors: John J Tudor; James J Davis; Marissa Panichella; Adam Zwolak
Journal: Appl Environ Microbiol Date: 2008-07-11 Impact factor: 4.792

8. Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria.

Authors: Rachel A Larsen; Marlena M Wilson; Adam M Guss; William W Metcalf
Journal: Arch Microbiol Date: 2002-06-18 Impact factor: 2.552

9. Population dynamics of Vibrio fischeri during infection of Euprymna scolopes.

Authors: Jessica McCann; Eric V Stabb; Deborah S Millikan; Edward G Ruby
Journal: Appl Environ Microbiol Date: 2003-10 Impact factor: 4.792

10. RdgB acts to avoid chromosome fragmentation in Escherichia coli.

Authors: Jill S Bradshaw; Andrei Kuzminov
Journal: Mol Microbiol Date: 2003-06 Impact factor: 3.501

28 in total

1. Host-selected mutations converging on a global regulator drive an adaptive leap towards symbiosis in bacteria.

Authors: M Sabrina Pankey; Randi L Foxall; Ian M Ster; Lauren A Perry; Brian M Schuster; Rachel A Donner; Matthew Coyle; Vaughn S Cooper; Cheryl A Whistler
Journal: Elife Date: 2017-04-27 Impact factor: 8.140

2. Role of Indole Production on Virulence of Vibrio cholerae Using Galleria mellonella Larvae Model.

Authors: Taiyeebah Nuidate; Natta Tansila; Suwat Saengkerdsub; Jetnaphang Kongreung; Dhamodharan Bakkiyaraj; Varaporn Vuddhakul
Journal: Indian J Microbiol Date: 2016-05-06 Impact factor: 2.461

3. Generation and In Vivo Characterization of Tn5-Induced Biofilm Mutants of Vibrio cholerae O139.

Authors: Preeti Gupta; Bharti Mankere; Shami Chekkoora Keloth; Urmil Tuteja; Kulanthaivel Thava Chelvam
Journal: Curr Microbiol Date: 2018-06-12 Impact factor: 2.188

4. O-antigen and core carbohydrate of Vibrio fischeri lipopolysaccharide: composition and analysis of their role in Euprymna scolopes light organ colonization.

Authors: Deborah M B Post; Liping Yu; Benjamin C Krasity; Biswa Choudhury; Mark J Mandel; Caitlin A Brennan; Edward G Ruby; Margaret J McFall-Ngai; Bradford W Gibson; Michael A Apicella
Journal: J Biol Chem Date: 2012-01-13 Impact factor: 5.157

5. An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.

Authors: Noreen L Lyell; Alecia N Septer; Anne K Dunn; Drew Duckett; Julie L Stoudenmire; Eric V Stabb
Journal: Appl Environ Microbiol Date: 2017-02-15 Impact factor: 4.792