Literature DB >> 18805413

Opisthorchis viverrini: detection by polymerase chain reaction (PCR) in human stool samples.

Kanasinakatte R Umesha1, Sanath Kumar, Ammini Parvathi, Kunyarat Duenngai, Paiboon Sithithaworn, Indrani Karunasagar, Iddya Karunasagar.   

Abstract

A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.

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Year:  2008        PMID： 18805413     DOI： 10.1016/j.exppara.2008.09.004

Source DB:  PubMed          Journal:  Exp Parasitol        ISSN： 0014-4894            Impact factor:   2.011


  10 in total

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10.  Improvement of a PCR-based method for the detection of Opisthorchis viverrini eggs in human stool samples by targeting internal transcribed spacer-2 (ITS-2), cytochrome oxidase subunit 1 (cox1), and cytochrome b (cyb).

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  10 in total

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