| Literature DB >> 18804353 |
César Marcos Marcello1, Andrei Stecca Steindorff, Silvana Petrofeza da Silva, Roberto do Nascimento Silva, Luiz Artur Mendes Bataus, Cirano José Ulhoa.
Abstract
The regulation of the gene encoding the extracellular exo-beta-1,3-glucanase (tag83) produced by the mycoparasite Trichoderma asperellum was studied. Enzyme activity was detected in all carbon sources, but the highest levels were found when starch and purified cell walls from Rhizoctonia solani were used. These results are supported by the appearance of one strong band with enzyme activity in non-denaturing PAGE. Experiments using RT-PCR showed that exo-beta-1,3-glucanase induction in T. asperellum occurred at the transcriptional level. We used RT-PCR and real-time PCR analysis to examine the expression of tag83 gene during in vivo assay of T. asperellum against R. solani. We showed that the expression of tag83 is significantly increased by the presence of R. solani.Entities:
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Year: 2008 PMID： 18804353 DOI： 10.1016/j.micres.2008.08.002
Source DB: PubMed Journal: Microbiol Res ISSN： 0944-5013 Impact factor: 5.415