| Literature DB >> 18802750 |
Aline Araujo dos Santos1, Simone Vidal Medina, Leandro de Araújo Martins, Elizabeth Giestal de Araujo.
Abstract
Protein kinase C (PKC) plays a key role in cellular events including proliferation, survival and differentiation. Our previous study showed the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, inducing a decrease in retinal cells proliferation. This effect was mediated by muscarinic type 1 receptors (M1) activation and brain derived neurotrophic factor (BDNF) treatment also induced a decrease in cell proliferation. Based on these results we analyzed the expression of either M1 receptors or BDNF following PMA treatment of retinal cell cultures. Our data demonstrated that PMA induced a decrease in both protein expressions after 48 h in culture. However, after 45 min, PMA induced a transient increase in BDNF expression and a decrease in M1 receptors expression. Analyzing the expression of M1 receptors and BDNF during the postnatal development in vivo, we observed a decrease in both proteins. Taken together our results suggest the involvement of PKC in the control of M1 expression in retinal cells.Entities:
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Year: 2008 PMID： 18802750 DOI： 10.1007/s11064-008-9847-x
Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996