BACKGROUND/AIMS: The TRPC1 gene on chromosome 3q22-24 resides within the linkage region for diabetic nephropathy (DN) in type 1 (T1D) and type 2 diabetes mellitus (T2D). A recent study has demonstrated that TRPC1 expression is reduced in the kidney of diabetic ZDF- and STZ-treated rats. The present study aimed to evaluate the genetic and functional role of TRPC1 in the development of DN. METHODS: Genetic association study was performed with two independent cohorts, including 1,177 T1D European Americans with or without DN from GoKinD population and 850 African-American subjects with T2D-associated end-stage renal disease (ESRD), or with hypertensive (non-diabetic) ESRD, and nondiabetic controls. Seven tag SNP markers derived from HapMap data (phase II) were genotyped. TRPC1 gene expression was examined using real time RT-PCR. RESULTS: No significant association of TRPC1 DNA polymorphisms with DN or ERSD was found in GoKinD and African-American populations. TRPC1 gene mRNA expression in kidney was found to be trendily reduced in 12-week and significantly in 26-week-old db/db mice. CONCLUSIONS: TRPC1 genetic polymorphism may not fundamentally contribute to the development of DN, while reduction of the gene expression in kidney may be a late phenomenon of DN as seen in diabetic animal models. 2008 S. Karger AG, Basel.
BACKGROUND/AIMS: The TRPC1 gene on chromosome 3q22-24 resides within the linkage region for diabetic nephropathy (DN) in type 1 (T1D) and type 2 diabetes mellitus (T2D). A recent study has demonstrated that TRPC1 expression is reduced in the kidney of diabeticZDF- and STZ-treated rats. The present study aimed to evaluate the genetic and functional role of TRPC1 in the development of DN. METHODS: Genetic association study was performed with two independent cohorts, including 1,177 T1D European Americans with or without DN from GoKinD population and 850 African-American subjects with T2D-associated end-stage renal disease (ESRD), or with hypertensive (non-diabetic) ESRD, and nondiabetic controls. Seven tag SNP markers derived from HapMap data (phase II) were genotyped. TRPC1 gene expression was examined using real time RT-PCR. RESULTS: No significant association of TRPC1 DNA polymorphisms with DN or ERSD was found in GoKinD and African-American populations. TRPC1 gene mRNA expression in kidney was found to be trendily reduced in 12-week and significantly in 26-week-old db/db mice. CONCLUSIONS:TRPC1 genetic polymorphism may not fundamentally contribute to the development of DN, while reduction of the gene expression in kidney may be a late phenomenon of DN as seen in diabetic animal models. 2008 S. Karger AG, Basel.
Authors: Donald W Bowden; Carla J Colicigno; Carl D Langefeld; Michèle M Sale; Adrienne Williams; Pamela J Anderson; Stephen S Rich; Barry I Freedman Journal: Kidney Int Date: 2004-10 Impact factor: 10.612
Authors: Dimitry A Chistiakov; Kirill V Savost'anov; Marina V Shestakova; Lyudmila A Chugunova; Minara Sh Samkhalova; Ivan I Dedov; Valery V Nosikov Journal: Diabetes Res Clin Pract Date: 2004-10 Impact factor: 5.602
Authors: Marcus G Pezzolesi; Jan Skupien; Josyf C Mychaleckyj; James H Warram; Andrzej S Krolewski Journal: Semin Nephrol Date: 2010-03 Impact factor: 5.299