Highly sensitive spectrofluorimetric determination of trace amounts of prulifloxacin using the aluminium(III)-prulifloxacin system.

The fluorescence of the prulifloxacin (PUFX)-Al(III) system was investigated . Experiments indicated that the fluorescence intensity of prulifloxacin could be greatly enhanced by Al(III) and sensitized by sodium dodecylbenzene sulphonate (SDBS). Accordingly, a sensitive spectrofluorimetric method for the determination of prulifloxacin was established. While excited at 275 nm, the enhanced fluorescence intensity at 412 nm of the system (DeltaF) showed a good linear relationship with the concentration of prulifloxacin within the range 4.0 x 10(-8)-3.0 x 10(-6) mol/L. The regression equation was DeltaF = 9.83 + 10.8 x 10(7)c (mol/L); the correlation coefficient and detection limit (3sigma/k) were 0.99901 and 2.0 x 10(-8) mol/L, respectively. The proposed method has been successfully applied to determine prulifloxacin in real pharmaceutical samples. The luminescence mechanism of the system is also discussed in detail. (c) 2008 John Wiley & Sons, Ltd.