AIMS: To investigate the action of different polyphenolic compounds, extracted from red wine, grape marc and pine bark, on oral bacteria. METHODS AND RESULTS: The anti-microbial activity of extracts was examined by determining the Minimal Inhibitory Concentration and Minimal Bactericidal Concentration using the macro dilution broth technique. Their effect on the adherence was tested on growing cells of Streptococcus mutans on a glass surface and on a multi-species biofilm grown on saliva-coated hydroxyapatite discs. The effect on glucosyltransferase activity was analysed through the reductions in the overall reaction rate and the quantity of insoluble glucan (ISG) synthesized. Pine bark and grape marc extracts were the most effective inhibitors of the multi-species biofilm formation and of the ISG synthesis. CONCLUSION: The tested components showed an interesting anti-plaque activity in vitro. SIGNIFICANCE AND IMPACT OF THE STUDY: This is, to our knowledge, the first and the most complete report on the properties of wine and pine bark extracts that could be used for oral disease prevention purpose.
AIMS: To investigate the action of different polyphenolic compounds, extracted from red wine, grape marc and pine bark, on oral bacteria. METHODS AND RESULTS: The anti-microbial activity of extracts was examined by determining the Minimal Inhibitory Concentration and Minimal Bactericidal Concentration using the macro dilution broth technique. Their effect on the adherence was tested on growing cells of Streptococcus mutans on a glass surface and on a multi-species biofilm grown on saliva-coated hydroxyapatite discs. The effect on glucosyltransferase activity was analysed through the reductions in the overall reaction rate and the quantity of insoluble glucan (ISG) synthesized. Pine bark and grape marc extracts were the most effective inhibitors of the multi-species biofilm formation and of the ISG synthesis. CONCLUSION: The tested components showed an interesting anti-plaque activity in vitro. SIGNIFICANCE AND IMPACT OF THE STUDY: This is, to our knowledge, the first and the most complete report on the properties of wine and pine bark extracts that could be used for oral disease prevention purpose.
Authors: Eduardo José Crevelin; Soraya Carolina Caixeta; Herbert Júnior Dias; Milton Groppo; Wilson Roberto Cunha; Carlos Henrique Gomes Martins; Antônio Eduardo Miller Crotti Journal: Evid Based Complement Alternat Med Date: 2015-06-16 Impact factor: 2.629
Authors: Nathalya Isabel de Melo; Carlos Eduardo de Carvalho; Letícia Fracarolli; Wilson Roberto Cunha; Rodrigo Cassio Sola Veneziani; Carlos Henrique Gomes Martins; Antônio Eduardo Miller Crotti Journal: Braz J Microbiol Date: 2015-06-01 Impact factor: 2.476
Authors: A Ledda; S Hu; M R Cesarone; G Belcaro; M Dugall; B Feragalli; R Cotellese; M Hosoi; E Ippolito; M Corsi; R Luzzi Journal: Evid Based Complement Alternat Med Date: 2021-06-23 Impact factor: 2.629
Authors: Lamprini Karygianni; Ali Al-Ahmad; Aikaterini Argyropoulou; Elmar Hellwig; Annette C Anderson; Alexios L Skaltsounis Journal: Front Microbiol Date: 2016-01-14 Impact factor: 5.640