Literature DB >> 1879430

Interference with myosin subfragment-1 binding by site-directed mutagenesis of actin.

P Aspenström1, R Karlsson.   

Abstract

Three N-terminal double mutants of beta-actin expressed in the yeast Saccharomyces cerevisiae have been characterized with respect to DNase-I interaction, N-terminal post-translational modification, polymerizability and myosin subfragment-1 binding. The results strongly support earlier suggestions that the acidic residues at the N-terminus of actin are part of the myosin-binding site, while they seem to be of no importance for the other aspects of actin biochemistry tested. The suitability of this expression system for production of recombinant actin in general is discussed.

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Year:  1991        PMID： 1879430     DOI： 10.1111/j.1432-1033.1991.tb21045.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  13 in total

1.  Functional characterization of the human α-cardiac actin mutations Y166C and M305L involved in hypertrophic cardiomyopathy.

Authors:  Mirco Müller; Antonina Joanna Mazur; Elmar Behrmann; Ralph P Diensthuber; Michael B Radke; Zheng Qu; Christoph Littwitz; Stefan Raunser; Cora-Ann Schoenenberger; Dietmar J Manstein; Hans Georg Mannherz
Journal:  Cell Mol Life Sci       Date:  2012-05-29       Impact factor: 9.261

Review 2.  Mutant actin: a dead end?

Authors:  E S Hennessey; A Harrison; D R Drummond; J C Sparrow
Journal:  J Muscle Res Cell Motil       Date:  1992-04       Impact factor: 2.698

3.  Small segmental rearrangements in the myosin head can explain force generation in muscle.

Authors:  F G Díaz Baños; J Bordas; J Lowy; A Svensson
Journal:  Biophys J       Date:  1996-08       Impact factor: 4.033

4.  Long-range conformational effects of proteolytic removal of the last three residues of actin.

Authors:  H Strzelecka-Gołaszewska; M Mossakowska; A Woźniak; J Moraczewska; H Nakayama
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857

5.  Charge-reversion mutagenesis of Dictyostelium actin to map the surface recognized by myosin during ATP-driven sliding motion.

Authors:  M Johara; Y Y Toyoshima; A Ishijima; H Kojima; T Yanagida; K Sutoh
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205

6.  Three-dimensional reconstruction of a co-complex of F-actin with antibody Fab fragments to actin's NH2 terminus.

Authors:  A Orlova; X Yu; E H Egelman
Journal:  Biophys J       Date:  1994-02       Impact factor: 4.033

Review 7.  Molecular genetics of actin function.

Authors:  E S Hennessey; D R Drummond; J C Sparrow
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

8.  Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants.

Authors:  J Buzan; J Du; T Karpova; C Frieden
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-16       Impact factor: 11.205

9.  Actomyosin kinetics and in vitro motility of wild-type Drosophila actin and the effects of two mutations in the Act88F gene.

Authors:  M Anson; D R Drummond; M A Geeves; E S Hennessey; M D Ritchie; J C Sparrow
Journal:  Biophys J       Date:  1995-05       Impact factor: 4.033

10.  Differential epitope tagging of actin in transformed Drosophila produces distinct effects on myofibril assembly and function of the indirect flight muscle.

Authors:  V Brault; U Sauder; M C Reedy; U Aebi; C A Schoenenberger
Journal:  Mol Biol Cell       Date:  1999-01       Impact factor: 4.138
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