OBJECTIVE: To identify the proteins displaying differential expression in midsecretory phase endometrium as compared with proliferative phase endometrium. DESIGN: Prospective study with two groups of women in the midsecretory or proliferative phase. SETTING: Clinical research outpatient department. PATIENT(S): Healthy, regularly cycling women of proven fertility. INTERVENTION(S): Collection of endometrial biopsy samples. MAIN OUTCOME MEASURE(S): Image analysis software was used to compare two-dimensional protein maps of midsecretory phase endometrial tissues (MSE) with maps of proliferative phase endometrial tissues (PROE) and midsecretory phase uterine fluids (MSU). Matrix-assisted laser desorption/ionization time of flight in tandem (MALDI-TOF-TOF) analysis was carried out to identify eight proteins that were differentially expressed between the two phases and also to identify the spots that shared similar coordinates in the two-dimensional maps of MSE and MSU. RESULT(S): Densitometry analysis and subsequent MALDI-TOF-TOF analysis revealed up-regulation of calreticulin, the beta chain of fibrinogen, adenylate kinase isoenzyme 5, and transferrin in the PROE and of annexin V, alpha1-antitrypsin, creatine kinase, and peroxidoxin 6 in MSE compared with the other phase. Superimposition of the two-dimensional maps of MSE on those of MSU revealed the presence of heat-shock protein 27, transferrin, and alpha1-antitrypsin precursor in both endometrial tissues and uterine secretions. CONCLUSION(S): Differentially expressed proteins identified in the present study could be of relevance in endowing the endometrium with receptivity.
OBJECTIVE: To identify the proteins displaying differential expression in midsecretory phase endometrium as compared with proliferative phase endometrium. DESIGN: Prospective study with two groups of women in the midsecretory or proliferative phase. SETTING: Clinical research outpatient department. PATIENT(S): Healthy, regularly cycling women of proven fertility. INTERVENTION(S): Collection of endometrial biopsy samples. MAIN OUTCOME MEASURE(S): Image analysis software was used to compare two-dimensional protein maps of midsecretory phase endometrial tissues (MSE) with maps of proliferative phase endometrial tissues (PROE) and midsecretory phase uterine fluids (MSU). Matrix-assisted laser desorption/ionization time of flight in tandem (MALDI-TOF-TOF) analysis was carried out to identify eight proteins that were differentially expressed between the two phases and also to identify the spots that shared similar coordinates in the two-dimensional maps of MSE and MSU. RESULT(S): Densitometry analysis and subsequent MALDI-TOF-TOF analysis revealed up-regulation of calreticulin, the beta chain of fibrinogen, adenylate kinase isoenzyme 5, and transferrin in the PROE and of annexin V, alpha1-antitrypsin, creatine kinase, and peroxidoxin 6 in MSE compared with the other phase. Superimposition of the two-dimensional maps of MSE on those of MSU revealed the presence of heat-shock protein 27, transferrin, and alpha1-antitrypsin precursor in both endometrial tissues and uterine secretions. CONCLUSION(S): Differentially expressed proteins identified in the present study could be of relevance in endowing the endometrium with receptivity.
Authors: Ioanna Kosteria; Athanasios K Anagnostopoulos; Christina Kanaka-Gantenbein; George P Chrousos; George T Tsangaris Journal: In Vivo Date: 2017 May-Jun Impact factor: 2.155
Authors: Jenifer H Anklesaria; Dhanashree D Jagtap; Bhakti R Pathak; Kaushiki M Kadam; Shaini Joseph; Smita D Mahale Journal: PLoS One Date: 2013-03-04 Impact factor: 3.240