OBJECTIVES: To evaluate biomarkers of endothelial dysfunction and oxidative stress in glucose intolerance (GI) compared to overt diabetes (DM2). DESIGN AND METHODS: 140 volunteers including 96 with DM2, 32 with GI and 12 controls (C) were studied. (*)NO metabolites, (*)NO synthase inhibitors, thiols and N-acetyl-beta-glucosaminidase (NAGase) activity were analyzed by chemiluminescence, capillary electrophoresis, ELISA and colorimetric assay, respectively. RESULTS: (*)NO metabolites were higher in GI (NOx: p=0.03; S-nitrosothiols: p=0.001) and DM2 (p=0.006; p=0.0006) groups in relation to group C, while nitrotyrosine was higher only in the DM2 group in comparison to the other groups. NAGase activity was elevated in GI (p=0.003) and DM2 (p=0.0004) groups in relation to group C, as well as, ADMA (p=0.01; p=0.003) and GSSG (p=0.01; p=0.002). CONCLUSIONS: (*)NO metabolites, (*)NO synthase inhibitors, thiols and NAGase are biomarkers suitable to indicate endothelial dysfunction and oxidative stress in the early stages of impaired response to insulin.
OBJECTIVES: To evaluate biomarkers of endothelial dysfunction and oxidative stress in glucose intolerance (GI) compared to overt diabetes (DM2). DESIGN AND METHODS: 140 volunteers including 96 with DM2, 32 with GI and 12 controls (C) were studied. (*)NO metabolites, (*)NO synthase inhibitors, thiols and N-acetyl-beta-glucosaminidase (NAGase) activity were analyzed by chemiluminescence, capillary electrophoresis, ELISA and colorimetric assay, respectively. RESULTS: (*)NO metabolites were higher in GI (NOx: p=0.03; S-nitrosothiols: p=0.001) and DM2 (p=0.006; p=0.0006) groups in relation to group C, while nitrotyrosine was higher only in the DM2 group in comparison to the other groups. NAGase activity was elevated in GI (p=0.003) and DM2 (p=0.0004) groups in relation to group C, as well as, ADMA (p=0.01; p=0.003) and GSSG (p=0.01; p=0.002). CONCLUSIONS: (*)NO metabolites, (*)NO synthase inhibitors, thiols and NAGase are biomarkers suitable to indicate endothelial dysfunction and oxidative stress in the early stages of impaired response to insulin.
Authors: An Pan; Qi Sun; Adam M Bernstein; Matthias B Schulze; JoAnn E Manson; Walter C Willett; Frank B Hu Journal: Am J Clin Nutr Date: 2011-08-10 Impact factor: 7.045
Authors: An Pan; Qi Sun; Adam M Bernstein; Matthias B Schulze; JoAnn E Manson; Meir J Stampfer; Walter C Willett; Frank B Hu Journal: Arch Intern Med Date: 2012-03-12
Authors: Thomas Forst; Andreas Pfützner; Frank Flacke; Alan Krasner; Cloth Hohberg; Eda Tarakci; Philip Pichotta; Senait Forst; Solomon Steiner Journal: Diabetes Care Date: 2009-10-06 Impact factor: 19.112