Literature DB >> 18791021

Evaluating the insecticidal genes and their expressed products in Bacillus thuringiensis strains by combining PCR with mass spectrometry.

Yunjun Sun1, Zujiao Fu, Xuezhi Ding, Liqiu Xia.   

Abstract

By a combination of PCR and mass spectrometry, a total of five cry genes (cry1Aa, cry1Ac, cry2Aa, cry2Ab, and cry1Ia) were detected in genomic DNA from the wild-type Bacillus thuringiensis strain 4.0718, and three protoxins (Cry1Aa, Cry1Ac, and Cry2Aa) were identified in the strain's parasporal crystals. These results indicated that this complementary method may be useful in evaluating B. thuringiensis strains at both the gene and protein levels.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18791021      PMCID： PMC2576694          DOI： 10.1128/AEM.01085-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  Characterization of Bacillus thuringiensis strain Bt185 toxic to the Asian cockchafer: Holotrichia parallela.

Authors:  Hong Yu; Jie Zhang; Dafang Huang; JiGuo Gao; Fuping Song
Journal:  Curr Microbiol       Date:  2006-06-09       Impact factor: 2.188

2.  From dormant to germinating spores of Streptomyces coelicolor A3(2): new perspectives from the crp null mutant.

Authors:  André Piette; Adeline Derouaux; Pascal Gerkens; Elke E E Noens; Gabriel Mazzucchelli; Sébastien Vion; Henk K Koerten; Fritz Titgemeyer; Edwin De Pauw; Pierre Leprince; Gilles P van Wezel; Moreno Galleni; Sébastien Rigali
Journal:  J Proteome Res       Date:  2005 Sep-Oct       Impact factor: 4.466

3.  Molecular and insecticidal characterization of a Bacillus thuringiensis strain isolated during a natural epizootic.

Authors:  M Porcar; P Caballero
Journal:  J Appl Microbiol       Date:  2000-08       Impact factor: 3.772

4.  Mass spectrometric sequencing of endotoxin proteins of Bacillus thuringiensis ssp. konkukian extracted from polyacrylamide gels.

Authors:  Kwang Yong Lee; Eun Young Kang; Sooil Park; Seong Kyu Ahn; Kwan Hee Yoo; Jin Young Kim; Hyung Hoan Lee
Journal:  Proteomics       Date:  2006-03       Impact factor: 3.984

Review 5.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

6.  A holistic approach for determining the entomopathogenic potential of Bacillus thuringiensis strains.

Authors:  L Masson; M Erlandson; M Puzstai-Carey; R Brousseau; V Juárez-Pérez; R Frutos
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

7.  Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection.

Authors:  A Bravo; S Sarabia; L Lopez; H Ontiveros; C Abarca; A Ortiz; M Ortiz; L Lina; F J Villalobos; G Peña; M E Nuñez-Valdez; M Soberón; R Quintero
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

8.  Specific PCR primers directed to identify cryI and cryIII genes within a Bacillus thuringiensis strain collection.

Authors:  J Cerón; A Ortíz; R Quintero; L Güereca; A Bravo
Journal:  Appl Environ Microbiol       Date:  1995-11       Impact factor: 4.792

9.  Isolation and characterization of a strain of Bacillus thuringiensis subsp. morrisoni PG-14 encoding delta-endotoxin Cry1Ac.

Authors:  Yong Soo Choi; Eun Sook Cho; Yeon Ho Je; Jong Yul Roh; Jin Hee Chang; Ming Shun Li; Sook Jae Seo; Hung Dae Sohn; Byung Rae Jin
Journal:  Curr Microbiol       Date:  2004-01       Impact factor: 2.188

10.  Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1I-type gene.

Authors:  Fuping Song; Jie Zhang; Aixing Gu; Yue Wu; Lanlan Han; Kanglai He; Zhongyi Chen; Jiang Yao; Yuqin Hu; Guoxun Li; Dafang Huang
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792
View more
  5 in total

1.  Resistance of Trichoplusia ni populations selected by Bacillus thuringiensis sprays to cotton plants expressing pyramided Bacillus thuringiensis toxins Cry1Ac and Cry2Ab.

Authors:  Wendy Kain; Xiaozhao Song; Alida F Janmaat; Jian-Zhou Zhao; Judith Myers; Anthony M Shelton; Ping Wang
Journal:  Appl Environ Microbiol       Date:  2014-12-05       Impact factor: 4.792

2.  Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry.

Authors:  Wenfei Zhang; Jie Zhang; Neil Crickmore; Zhongqi Wu; Yiran Yang; Jiangzhao Qian; Hongping Wu; Ruiping Wang; Xuanjun Fang
Journal:  World J Microbiol Biotechnol       Date:  2014-09-26       Impact factor: 3.312

3.  Proteomic analysis of Bacillus thuringiensis at different growth phases by using an automated online two-dimensional liquid chromatography-tandem mass spectrometry strategy.

Authors:  Shaoya Huang; Xuezhi Ding; Yunjun Sun; Qi Yang; Xiuqing Xiao; Zhenping Cao; Liqiu Xia
Journal:  Appl Environ Microbiol       Date:  2012-05-25       Impact factor: 4.792

4.  Detection of toxin proteins from Bacillus thuringiensis strain 4.0718 by strategy of 2D-LC-MS/MS.

Authors:  Qi Yang; Sijia Tang; Jie Rang; Mingxing Zuo; Xuezhi Ding; Yunjun Sun; Pinghui Feng; Liqiu Xia
Journal:  Curr Microbiol       Date:  2014-12-05       Impact factor: 2.188

5.  Genomics and Proteomics Analyses Revealed Novel Candidate Pesticidal Proteins in a Lepidopteran-Toxic Bacillus thuringiensis Strain.

Authors:  Ayda Khorramnejad; Joaquín Gomis-Cebolla; Reza Talaei-Hassanlouei; Yolanda Bel; Baltasar Escriche
Journal:  Toxins (Basel)       Date:  2020-10-26       Impact factor: 4.546
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.