BACKGROUND:Acute bacterial meningitis is a significant cause of morbidity and mortality throughout the world. It can be difficult to diagnose, as the symptoms and signs are often non-specific. STUDY OBJECTIVE: To evaluate the performance of an in-house semi-nested polymerase chain reaction (PCR) assay targeting the 16S rRNA gene of Eubacteria for the rapid diagnosis of acute bacterial meningitis using cerebrospinal fluid (CSF) specimens. METHODS:A total of 112 CSF samples from 112 patients were used in the study. Among these, 32 samples were obtained from confirmed cases of Streptococcus pneumoniae, six samples were obtained from confirmed cases of Haemophilus influenzae, one sample from a confirmed case of Neisseria meningitidis, and 10 cases of clinically suspected acute bacterial meningitis. The remaining 63 CSF samples were obtained from patients with non-infectious illnesses (n = 47) of the central nervous system (CNS) and autopsy-confirmed tuberculous meningitis (n = 16). RESULTS: The assay had an overall sensitivity of 93% (95% confidence interval [CI] 0.81-0.98, negative predictive value = 95%) and a specificity of 98% (95% CI 0.92-1.0, positive predictive value = 98%). CONCLUSION: These preliminary findings suggest that the semi-nested PCR assay targeting the 16S rRNA gene may be used as a rapid test for the diagnosis of acute bacterial meningitis. Copyright (c) 2010 Elsevier Inc. All rights reserved.
RCT Entities:
BACKGROUND: Acute bacterial meningitis is a significant cause of morbidity and mortality throughout the world. It can be difficult to diagnose, as the symptoms and signs are often non-specific. STUDY OBJECTIVE: To evaluate the performance of an in-house semi-nested polymerase chain reaction (PCR) assay targeting the 16S rRNA gene of Eubacteria for the rapid diagnosis of acute bacterial meningitis using cerebrospinal fluid (CSF) specimens. METHODS: A total of 112 CSF samples from 112 patients were used in the study. Among these, 32 samples were obtained from confirmed cases of Streptococcus pneumoniae, six samples were obtained from confirmed cases of Haemophilus influenzae, one sample from a confirmed case of Neisseria meningitidis, and 10 cases of clinically suspected acute bacterial meningitis. The remaining 63 CSF samples were obtained from patients with non-infectious illnesses (n = 47) of the central nervous system (CNS) and autopsy-confirmed tuberculous meningitis (n = 16). RESULTS: The assay had an overall sensitivity of 93% (95% confidence interval [CI] 0.81-0.98, negative predictive value = 95%) and a specificity of 98% (95% CI 0.92-1.0, positive predictive value = 98%). CONCLUSION: These preliminary findings suggest that the semi-nested PCR assay targeting the 16S rRNA gene may be used as a rapid test for the diagnosis of acute bacterial meningitis. Copyright (c) 2010 Elsevier Inc. All rights reserved.
Authors: Richard Rothman; Padmini Ramachandran; Samuel Yang; Andrew Hardick; Helen Won; Aleksandar Kecojevic; Celeste Quianzon; Yu-Hsiang Hsieh; Charlotte Gaydos Journal: Acad Emerg Med Date: 2010-07 Impact factor: 3.451
Authors: Soumitesh Chakravorty; Bola Aladegbami; Michele Burday; Michael Levi; Salvatore A E Marras; Darshini Shah; Hiyam H El-Hajj; Fred Russell Kramer; David Alland Journal: J Clin Microbiol Date: 2009-11-18 Impact factor: 5.948
Authors: T Meyer; G Franke; S K A Polywka; M Lütgehetmann; J Gbadamosi; T Magnus; M Aepfelbacher Journal: J Clin Microbiol Date: 2014-03-12 Impact factor: 5.948
Authors: A Pormohammad; S Lashkarbolouki; T Azimi; P Gholizadeh; N Bostanghadiri; H Safari; S Armin; N Mohtavinejad; F Fallah Journal: New Microbes New Infect Date: 2019-08-29