PURPOSE: This project explored the influence of loss of heterozygosity (LOH) and microsatellite instability (MSI) of locus D8S277 to PINX1 expression of gastric cancer in Chinese people. METHODS: LOH and MSI of locus D8S277 in 90 paraffin-embedded gastric carcinoma specimens were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Envision immunohistochemistry was used to assess the expression of PINX1. RESULTS: The frequency of LOH was higher in cases with lymph node metastasis than those without (18.57 vs. 0.00%, P < 0.01), and was higher in the specimens that were at TNM stage III + IV than those at stage I + II (21.43 vs. 2.94%, P < 0.01). In terms of the frequency of MSI, it was lower in cases with lymph node metastasis than those without (10.00 vs. 30.00%,P < 0.05). The positive rate of PINX1 protein was higher in samples without lymph node metastasis than those with lymph node metastasis (80.00 vs. 50.00%, P < 0.01); and was higher in the cases at TNM stage I + II than those at stage III + IV; and was lower in the cases between 40 and 60 years old than those above 60 years old (43.75 vs. 65.52%, P < 0.05). CONCLUSION: LOH and MSI of PINX1 may play major role in tumor development and regulate it through different pathways. Because LOH plays a major role in negative expression of PINX1, it can be regarded as a sign of gastric cancer development and MSI may affect the prognosis and tumor turnover.
PURPOSE: This project explored the influence of loss of heterozygosity (LOH) and microsatellite instability (MSI) of locus D8S277 to PINX1 expression of gastric cancer in Chinese people. METHODS: LOH and MSI of locus D8S277 in 90 paraffin-embedded gastric carcinoma specimens were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Envision immunohistochemistry was used to assess the expression of PINX1. RESULTS: The frequency of LOH was higher in cases with lymph node metastasis than those without (18.57 vs. 0.00%, P < 0.01), and was higher in the specimens that were at TNM stage III + IV than those at stage I + II (21.43 vs. 2.94%, P < 0.01). In terms of the frequency of MSI, it was lower in cases with lymph node metastasis than those without (10.00 vs. 30.00%,P < 0.05). The positive rate of PINX1 protein was higher in samples without lymph node metastasis than those with lymph node metastasis (80.00 vs. 50.00%, P < 0.01); and was higher in the cases at TNM stage I + II than those at stage III + IV; and was lower in the cases between 40 and 60 years old than those above 60 years old (43.75 vs. 65.52%, P < 0.05). CONCLUSION: LOH and MSI of PINX1 may play major role in tumor development and regulate it through different pathways. Because LOH plays a major role in negative expression of PINX1, it can be regarded as a sign of gastric cancer development and MSI may affect the prognosis and tumor turnover.
Authors: Won Sang Park; Jong Heun Lee; Jik Young Park; Seong Whan Jeong; Min Sun Shin; Hong Sug Kim; Sang Kyu Lee; Shi Nae Lee; Sug Hyung Lee; Chul Geun Park; Nam Jin Yoo; Jung Young Lee Journal: Cancer Lett Date: 2002-04-25 Impact factor: 8.679
Authors: M Emi; Y Fujiwara; T Nakajima; E Tsuchiya; H Tsuda; S Hirohashi; Y Maeda; K Tsuruta; M Miyaki; Y Nakamura Journal: Cancer Res Date: 1992-10-01 Impact factor: 12.701
Authors: Gregory A Hawkins; Bao-Li Chang; S Lilly Zheng; Sarah D Isaacs; Kathleen E Wiley; Eugene R Bleecker; Patrick C Walsh; Deborah A Meyers; Jianfeng Xu; William B Isaacs Journal: Prostate Date: 2004-09-01 Impact factor: 4.104
Authors: M Konishi; R Kikuchi-Yanoshita; K Tanaka; C Sato; K Tsuruta; Y Maeda; M Koike; S Tanaka; Y Nakamura; N Hattori Journal: Jpn J Cancer Res Date: 1993-08