OBJECTIVES: To evaluate the in vivo antimicrobial activity on the salivary flora of a single mouthrinse of chlorhexidine (CHX) digluconate, analysing the influence of its concentration (0.2% versus 0.12%). METHODS: The study group was formed of 20 adult volunteers with a good oral health status. Non-stimulated saliva samples were collected under basal conditions and at 30s and 1h after a single mouthrinse with sterile water, 0.2% or 0.12% CHX digluconate. Serial dilutions were then performed and the resulting samples were cultured on conventional culture media for aerobes/facultative anaerobes and obligate anaerobes. The number of colony forming units (CFU/ml) was then determined and the results expressed on a decimal log scale (log(10)CFU/ml). RESULTS: A significant reduction in the total bacterial population was observed at 30s and 1h after the mouthrinse with both CHX concentrations; this antimicrobial activity was more pronounced on the obligate anaerobes. The antimicrobial activity of 0.2% CHX on the salivary flora at 30s and 1h after the mouthrinse was significantly greater than that of 0.12% CHX. Only 0.2% CHX showed bactericidal activity (differential factor> or =3 log(10)CFU/ml) against salivary obligate anaerobes. CONCLUSION: The greater antimicrobial activity of 0.2% CHX confirms the influence of the concentration on its antibacterial activity. In consequence, the CHX concentration seems to be an important factor to guarantee a high antibacterial activity in those clinical situations where it is required.
OBJECTIVES: To evaluate the in vivo antimicrobial activity on the salivary flora of a single mouthrinse of chlorhexidine (CHX) digluconate, analysing the influence of its concentration (0.2% versus 0.12%). METHODS: The study group was formed of 20 adult volunteers with a good oral health status. Non-stimulated saliva samples were collected under basal conditions and at 30s and 1h after a single mouthrinse with sterile water, 0.2% or 0.12% CHX digluconate. Serial dilutions were then performed and the resulting samples were cultured on conventional culture media for aerobes/facultative anaerobes and obligate anaerobes. The number of colony forming units (CFU/ml) was then determined and the results expressed on a decimal log scale (log(10)CFU/ml). RESULTS: A significant reduction in the total bacterial population was observed at 30s and 1h after the mouthrinse with both CHX concentrations; this antimicrobial activity was more pronounced on the obligate anaerobes. The antimicrobial activity of 0.2% CHX on the salivary flora at 30s and 1h after the mouthrinse was significantly greater than that of 0.12% CHX. Only 0.2% CHX showed bactericidal activity (differential factor> or =3 log(10)CFU/ml) against salivary obligate anaerobes. CONCLUSION: The greater antimicrobial activity of 0.2% CHX confirms the influence of the concentration on its antibacterial activity. In consequence, the CHX concentration seems to be an important factor to guarantee a high antibacterial activity in those clinical situations where it is required.
Authors: O A González; C Escamilla; R J Danaher; J Dai; J L Ebersole; R J Mumper; C S Miller Journal: J Periodontal Res Date: 2012-07-19 Impact factor: 4.419
Authors: Christiane von Ohle; Armin Gieseke; Laura Nistico; Eva Maria Decker; Dirk DeBeer; Paul Stoodley Journal: Appl Environ Microbiol Date: 2010-01-29 Impact factor: 4.792
Authors: Victor Quintas; Isabel Prada-López; María J Carreira; David Suárez-Quintanilla; Carlos Balsa-Castro; Inmaculada Tomás Journal: Front Microbiol Date: 2017-11-23 Impact factor: 5.640