BACKGROUND: Antiphospholipid antibodies (aPL) are considered to be a cause of an acquired hypercoagulable state leading to cerebral infarction (CI). Apolipoprtein H (apoH) is an important target antigen for aPL and thus apoH polymorphisms may influence aPL production and the development of CI. The purpose of this study was to identify associations between the Val/Leu(247) polymorphism of apoH gene and CI in a Chinese cohort. METHODS: This study comprised 130 CI patients and 100 healthy control subjects. Polymorphism assignment was determined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and DNA sequencing. The presence of aPL was detected by ELISA utilizing irradiated ELISA plates. RESULTS: Our results demonstrated an association between the Val/Leu(247) polymorphism of apoH gene and aPL in CI patients. The frequency of V allele was significantly higher in aPL-positive CI patients compared with control group (chi(2) = 6.864, P < 0.05). VL genotype frequency was also significantly higher in aPL-positive CI group compared with control group (chi(2) = 13.879, P < 0.05) and aPL-negative CI group (chi(2) = 5.567, P < 0.05). CONCLUSIONS: The Val(247) allele of apoH gene is significantly associated with the presence of aPL in Chinese patients with CI.
BACKGROUND: Antiphospholipid antibodies (aPL) are considered to be a cause of an acquired hypercoagulable state leading to cerebral infarction (CI). Apolipoprtein H (apoH) is an important target antigen for aPL and thus apoH polymorphisms may influence aPL production and the development of CI. The purpose of this study was to identify associations between the Val/Leu(247) polymorphism of apoH gene and CI in a Chinese cohort. METHODS: This study comprised 130 CI patients and 100 healthy control subjects. Polymorphism assignment was determined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique and DNA sequencing. The presence of aPL was detected by ELISA utilizing irradiated ELISA plates. RESULTS: Our results demonstrated an association between the Val/Leu(247) polymorphism of apoH gene and aPL in CI patients. The frequency of V allele was significantly higher in aPL-positive CI patients compared with control group (chi(2) = 6.864, P < 0.05). VL genotype frequency was also significantly higher in aPL-positive CI group compared with control group (chi(2) = 13.879, P < 0.05) and aPL-negative CI group (chi(2) = 5.567, P < 0.05). CONCLUSIONS: The Val(247) allele of apoH gene is significantly associated with the presence of aPL in Chinese patients with CI.
Authors: G J Hademenos; M J Alberts; I Awad; M Mayberg; T Shepard; A Jagoda; R E Latchaw; H W Todd; K Viste; R Starke; M S Girgus; J Marler; M Emr; N Hart Journal: Neurology Date: 2001-04-24 Impact factor: 9.910
Authors: Haider Mehdi; Susan Manzi; Purnima Desai; Qi Chen; Cara Nestlerode; Franklin Bontempo; Stephen C Strom; Reza Zarnegar; M Ilyas Kamboh Journal: Eur J Biochem Date: 2003-01
Authors: G Aleph Prieto; Antonio R Cabral; Martín Zapata-Zuñiga; Abraham J Simón; Antonio R Villa; Donato Alarcón-Segovia; Javier Cabiedes Journal: Arthritis Rheum Date: 2003-02
Authors: M Ilyas Kamboh; Dharambir K Sanghera; Haider Mehdi; Cara S Nestlerode; Qi Chen; Osama Khalifa; Asma Naqvi; Susan Manzi; Clareann H Bunker Journal: Ann Hum Genet Date: 2004-07 Impact factor: 1.670