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Literature DB >> 18775698

Oxidative demethylation of 3-methylthymine and 3-methyluracil in single-stranded DNA and RNA by mouse and human FTO.

Guifang Jia¹, Cai-Guang Yang, Shangdong Yang, Xing Jian, Chengqi Yi, Zhiqiang Zhou, Chuan He.

Abstract

The human obesity susceptibility gene, FTO, encodes a protein that is homologous to the DNA repair AlkB protein. The AlkB family proteins utilize iron(II), alpha-ketoglutarate (alpha-KG) and dioxygen to perform oxidative repair of alkylated nucleobases in DNA and RNA. We demonstrate here the oxidative demethylation of 3-methylthymine (3-meT) in single-stranded DNA (ssDNA) and 3-methyluracil (3-meU) in single-stranded RNA (ssRNA) by recombinant human FTO protein in vitro. Both human and mouse FTO proteins preferentially repair 3-meT in ssDNA over other base lesions tested. They showed negligible activities against 3-meT in double-stranded DNA (dsDNA). In addition, these two proteins can catalyze the demethylation of 3-meU in ssRNA with a slightly higher efficiency over that of 3-meT in ssDNA, suggesting that methylated RNAs are the preferred substrates for FTO.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 2008 PMID： 18775698 PMCID： PMC2577709 DOI： 10.1016/j.febslet.2008.08.019

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

29 in total

1. Preparation and enzymatic hydrolysis of DNA and RNA for mass spectrometry.

Authors: P F Crain
Journal: Methods Enzymol Date: 1990 Impact factor: 1.600

2. Reversal of DNA alkylation damage by two human dioxygenases.

Authors: Tod Duncan; Sarah C Trewick; Pertti Koivisto; Paul A Bates; Tomas Lindahl; Barbara Sedgwick
Journal: Proc Natl Acad Sci U S A Date: 2002-12-16 Impact factor: 11.205

3. Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA.

Authors: Per Arne Aas; Marit Otterlei; Pål O Falnes; Cathrine B Vågbø; Frank Skorpen; Mansour Akbari; Ottar Sundheim; Magnar Bjørås; Geir Slupphaug; Erling Seeberg; Hans E Krokan
Journal: Nature Date: 2003-02-20 Impact factor: 49.962

4. Repair of 3-methylthymine and 1-methylguanine lesions by bacterial and human AlkB proteins.

Authors: Pål Ø Falnes
Journal: Nucleic Acids Res Date: 2004-12-01 Impact factor: 16.971

5. Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli.

Authors: James C Delaney; John M Essigmann
Journal: Proc Natl Acad Sci U S A Date: 2004-09-20 Impact factor: 11.205

6. Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage.

Authors: Sarah C Trewick; Timothy F Henshaw; Robert P Hausinger; Tomas Lindahl; Barbara Sedgwick
Journal: Nature Date: 2002-09-12 Impact factor: 49.962

7. Substrate specificities of bacterial and human AlkB proteins.

Authors: Pål Ø Falnes; Magnar Bjørås; Per Arne Aas; Ottar Sundheim; Erling Seeberg
Journal: Nucleic Acids Res Date: 2004-06-30 Impact factor: 16.971

8. Incorporation of oxygen into the succinate co-product of iron(II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans.

Authors: Richard W D Welford; Joanna M Kirkpatrick; Luke A McNeill; Munish Puri; Neil J Oldham; Christopher J Schofield
Journal: FEBS Lett Date: 2005-09-26 Impact factor: 4.124

9. Phylogenomic identification of five new human homologs of the DNA repair enzyme AlkB.

Authors: Michal A Kurowski; Ashok S Bhagwat; Grzegorz Papaj; Janusz M Bujnicki
Journal: BMC Genomics Date: 2003-12-10 Impact factor: 3.969

10. The selectivity and inhibition of AlkB.

Authors: Richard W D Welford; Imre Schlemminger; Luke A McNeill; Kirsty S Hewitson; Christopher J Schofield
Journal: J Biol Chem Date: 2003-01-06 Impact factor: 5.157

147 in total

Review 1. A loop matters for FTO substrate selection.

Authors: Zhifu Han; Ning Huang; Tianhui Niu; Jijie Chai
Journal: Protein Cell Date: 2010-07-29 Impact factor: 14.870

2. Maternal and neonatal FTO rs9939609 polymorphism affect insulin sensitivity markers and lipoprotein profile at birth in appropriate-for-gestational-age term neonates.

Authors: Eva Gesteiro; Francisco J Sánchez-Muniz; Carolina Ortega-Azorín; Marisa Guillén; Dolores Corella; Sara Bastida
Journal: J Physiol Biochem Date: 2016-02-06 Impact factor: 4.158

3. Cut-like homeobox 1 (CUX1) regulates expression of the fat mass and obesity-associated and retinitis pigmentosa GTPase regulator-interacting protein-1-like (RPGRIP1L) genes and coordinates leptin receptor signaling.

Authors: George Stratigopoulos; Charles A LeDuc; Maria L Cremona; Wendy K Chung; Rudolph L Leibel
Journal: J Biol Chem Date: 2010-10-31 Impact factor: 5.157

4. Genome-wide association analysis of body mass in chronic obstructive pulmonary disease.

Authors: Emily S Wan; Michael H Cho; Nadia Boutaoui; Barbara J Klanderman; Jody S Sylvia; John P Ziniti; Sungho Won; Christoph Lange; Sreekumar G Pillai; Wayne H Anderson; Xiangyang Kong; David A Lomas; Per S Bakke; Amund Gulsvik; Elizabeth A Regan; James R Murphy; Barry J Make; James D Crapo; Emiel F Wouters; Bartolome R Celli; Edwin K Silverman; Dawn L DeMeo
Journal: Am J Respir Cell Mol Biol Date: 2010-10-29 Impact factor: 6.914

Review 10. The AlkB Family of Fe(II)/α-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond.

Authors: Bogdan I Fedeles; Vipender Singh; James C Delaney; Deyu Li; John M Essigmann
Journal: J Biol Chem Date: 2015-07-07 Impact factor: 5.157

Oxidative demethylation of 3-methylthymine and 3-methyluracil in single-stranded DNA and RNA by mouse and human FTO.

1. Preparation and enzymatic hydrolysis of DNA and RNA for mass spectrometry.

2. Reversal of DNA alkylation damage by two human dioxygenases.

3. Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA.

4. Repair of 3-methylthymine and 1-methylguanine lesions by bacterial and human AlkB proteins.

5. Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli.

6. Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage.

7. Substrate specificities of bacterial and human AlkB proteins.

8. Incorporation of oxygen into the succinate co-product of iron(II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans.

9. Phylogenomic identification of five new human homologs of the DNA repair enzyme AlkB.

10. The selectivity and inhibition of AlkB.

Review 1. A loop matters for FTO substrate selection.

2. Maternal and neonatal FTO rs9939609 polymorphism affect insulin sensitivity markers and lipoprotein profile at birth in appropriate-for-gestational-age term neonates.

3. Cut-like homeobox 1 (CUX1) regulates expression of the fat mass and obesity-associated and retinitis pigmentosa GTPase regulator-interacting protein-1-like (RPGRIP1L) genes and coordinates leptin receptor signaling.

4. Genome-wide association analysis of body mass in chronic obstructive pulmonary disease.

5. Uncovering the biology of FTO.

6. Association of FTO rs9939609 with Obesity in the Kuwaiti Population: A Public Health Concern?

Review 7. Damage detection and base flipping in direct DNA alkylation repair.

8. Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites.

Review 9. m⁶A RNA Methylation Controls Neural Development and Is Involved in Human Diseases.

Review 10. The AlkB Family of Fe(II)/α-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond.

Review 1. A loop matters for FTO substrate selection.

Review 7. Damage detection and base flipping in direct DNA alkylation repair.

Review 9. m6A RNA Methylation Controls Neural Development and Is Involved in Human Diseases.

Review 10. The AlkB Family of Fe(II)/α-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond.

Review 9. m⁶A RNA Methylation Controls Neural Development and Is Involved in Human Diseases.