Literature DB >> 18775482

High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene.

Kazufumi Mochizuki1.   

Abstract

Tetrahymena thermophila is a useful model for the study of eukaryotic biology. A neomycin resistance gene (neo) has been developed that was optimized for the codon usage of T. thermophila. Using this codon-optimized neo gene (neoTet), a new drug resistance marker cassette, neo4, has been constructed. The neo4 cassette resulted in about ten times more drug resistant transformants than a cassette containing the non-codon-optimized original neo gene. The new cassette enables transgenic Tetrahymena strains to be created with high efficiency. This study also emphasizes the importance of codon optimization in transgene expression in Tetrahymena.

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Year:  2008        PMID： 18775482     DOI： 10.1016/j.gene.2008.08.007

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  47 in total

1.  Tetrahymena Poc5 is a transient basal body component that is important for basal body maturation.

Authors:  Westley Heydeck; Brian A Bayless; Alexander J Stemm-Wolf; Eileen T O'Toole; Amy S Fabritius; Courtney Ozzello; Marina Nguyen; Mark Winey
Journal:  J Cell Sci       Date:  2020-06-04       Impact factor: 5.285

2.  Quantitative proteomics reveals histone modifications in crosstalk with H3 lysine 27 methylation.

Authors:  Chunchao Zhang; Shan Gao; Anthony J Molascon; Yifan Liu; Philip C Andrews
Journal:  Mol Cell Proteomics       Date:  2014-01-01       Impact factor: 5.911

3.  Tetrahymena thermophila JMJD3 homolog regulates H3K27 methylation and nuclear differentiation.

Authors:  Pei-Han Chung; Meng-Chao Yao
Journal:  Eukaryot Cell       Date:  2012-03-16

4.  Role of ATG8 and autophagy in programmed nuclear degradation in Tetrahymena thermophila.

Authors:  Ming-Liang Liu; Meng-Chao Yao
Journal:  Eukaryot Cell       Date:  2012-02-24

5.  Generating and Maintaining Transgenic Cryptosporidium parvum Parasites.

Authors:  Mattie C Pawlowic; Sumiti Vinayak; Adam Sateriale; Carrie F Brooks; Boris Striepen
Journal:  Curr Protoc Microbiol       Date:  2017-08-11

6.  Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila.

Authors:  Guan-Xiong Yan; Huai Dang; Miao Tian; Jing Zhang; Anura Shodhan; Ying-Zhi Ning; Jie Xiong; Wei Miao
Journal:  Cell Cycle       Date:  2016-05-18       Impact factor: 4.534

7.  E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila.

Authors:  Jing Zhang; Miao Tian; Guan-Xiong Yan; Anura Shodhan; Wei Miao
Journal:  Cell Cycle       Date:  2016-11-28       Impact factor: 4.534

8.  Modules for C-terminal epitope tagging of Tetrahymena genes.

Authors:  Kensuke Kataoka; Ursula E Schoeberl; Kazufumi Mochizuki
Journal:  J Microbiol Methods       Date:  2010-07-17       Impact factor: 2.363

9.  Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena.

Authors:  Clara Jana-Lui Busch; Alexander Vogt; Kazufumi Mochizuki
Journal:  BMC Microbiol       Date:  2010-07-13       Impact factor: 3.605

10.  MEC-17 is an alpha-tubulin acetyltransferase.

Authors:  Jyothi S Akella; Dorota Wloga; Jihyun Kim; Natalia G Starostina; Sally Lyons-Abbott; Naomi S Morrissette; Scott T Dougan; Edward T Kipreos; Jacek Gaertig
Journal:  Nature       Date:  2010-09-09       Impact factor: 49.962
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