OBJECTIVE: To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1(*)2C, and male factor infertility. DESIGN: Genetic polymorphism analysis, case-control study. SETTING: University research laboratory and andrology clinic. PATIENT(S): One hundred ten men with infertility and 105 healthy fertile men were recruited for the study. INTERVENTION(S): Physical examination of the genitalia of patients, scrotal colored Doppler ultrasound examination, and blood sampling were performed for DNA extraction and genotyping. MAIN OUTCOME MEASURE(S): CYP1A1(*)2C, GSTM1, and GSTT1 polymorphism genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism methods. Seminal parameters were analyzed. RESULT(S): There were significant differences between infertility and GSTM1, CYP1A1(*)2C genotypes by univariate analyses. A subject carrying CYP1A1 Val/Val or CYP1A1 Ile/Val in association with GSTM null genotype has 6.90 times more risk to be infertile than a subject carrying CYP1A1 Ile/Ile in association with GSTM1 wild-type genotype (odds ratio: 6.90, 95% confidence interval: 2.29-19.3). No correlation was found between the seminal parameters and the genetic variability. CONCLUSION: Our results suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in infertility.
OBJECTIVE: To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1(*)2C, and male factor infertility. DESIGN: Genetic polymorphism analysis, case-control study. SETTING: University research laboratory and andrology clinic. PATIENT(S): One hundred ten men with infertility and 105 healthy fertile men were recruited for the study. INTERVENTION(S): Physical examination of the genitalia of patients, scrotal colored Doppler ultrasound examination, and blood sampling were performed for DNA extraction and genotyping. MAIN OUTCOME MEASURE(S): CYP1A1(*)2C, GSTM1, and GSTT1 polymorphism genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism methods. Seminal parameters were analyzed. RESULT(S): There were significant differences between infertility and GSTM1, CYP1A1(*)2C genotypes by univariate analyses. A subject carrying CYP1A1 Val/Val or CYP1A1 Ile/Val in association with GSTM null genotype has 6.90 times more risk to be infertile than a subject carrying CYP1A1 Ile/Ile in association with GSTM1 wild-type genotype (odds ratio: 6.90, 95% confidence interval: 2.29-19.3). No correlation was found between the seminal parameters and the genetic variability. CONCLUSION: Our results suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in infertility.
Authors: Andrew F Olshan; Thomas J Luben; Nancy M Hanley; Sally D Perreault; Ronna L Chan; Amy H Herring; Patricia V Basta; David M DeMarini Journal: Mutat Res Date: 2010-03-07 Impact factor: 2.433