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Literature DB >> 18773150

Re-engineering Escherichia coli for ethanol production.

L P Yomano¹, S W York, S Zhou, K T Shanmugam, L O Ingram.

Abstract

A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and adhB) into the chromosome. By selecting for fermentative growth in mineral salts medium containing xylose, a highly productive strain was isolated in which the ethanol cassette had been integrated behind the rrlE promoter, designated strain LY160(KO11, Deltafrd::celY(Ec) DeltaadhE DeltaldhA, DeltaackA lacA::casAB(Ko) rrlE::(pdc( Zm)-adhA(Zm)-adhB(Zm)-FRT-rrlE)pflB(+)). This strain fermented 9% (w/v) xylose to 4% (w/v) ethanol in 48 h in mineral salts medium, nearly equal to the performance of KO11 with Luria broth.

Entities: Chemical Species

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Year: 2008 PMID： 18773150 DOI： 10.1007/s10529-008-9821-3

Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461

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Cited

41 in total

1. Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes.

Authors: Peter C Turner; Lorraine P Yomano; Laura R Jarboe; Sean W York; Christy L Baggett; Brélan E Moritz; Emily B Zentz; K T Shanmugam; Lonnie O Ingram
Journal: J Ind Microbiol Biotechnol Date: 2011-11-11 Impact factor: 3.346

2. Cell surface display of a β-glucosidase employing the type V secretion system on ethanologenic Escherichia coli for the fermentation of cellobiose to ethanol.

Authors: Iván Muñoz-Gutiérrez; Ricardo Oropeza; Guillermo Gosset; Alfredo Martinez
Journal: J Ind Microbiol Biotechnol Date: 2012-05-26 Impact factor: 3.346

3. Adaptive evolution of nontransgenic Escherichia coli KC01 for improved ethanol tolerance and homoethanol fermentation from xylose.

Authors: Yongze Wang; Ryan Manow; Christopher Finan; Jinhua Wang; Erin Garza; Shengde Zhou
Journal: J Ind Microbiol Biotechnol Date: 2010-12-29 Impact factor: 3.346

4. Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli.

Authors: E N Miller; L R Jarboe; L P Yomano; S W York; K T Shanmugam; L O Ingram
Journal: Appl Environ Microbiol Date: 2009-05-08 Impact factor: 4.792

5. Revealing the genetic basis of natural bacterial phenotypic divergence.

Authors: Peter L Freddolino; Hani Goodarzi; Saeed Tavazoie
Journal: J Bacteriol Date: 2013-12-06 Impact factor: 3.490

6. Escherichia coli strains engineered for homofermentative production of D-lactic acid from glycerol.

Authors: Suman Mazumdar; James M Clomburg; Ramon Gonzalez
Journal: Appl Environ Microbiol Date: 2010-05-14 Impact factor: 4.792

7. Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals.

Authors: Xuan Wang; Lorraine P Yomano; James Y Lee; Sean W York; Huabao Zheng; Michael T Mullinnix; K T Shanmugam; Lonnie O Ingram
Journal: Proc Natl Acad Sci U S A Date: 2013-02-19 Impact factor: 11.205