Literature DB >> 18770734

Analysis of DNA content and BrdU incorporation.

Z Darzynkiewicz1, G Juan.   

Abstract

During the past two decades, the techniques of analysis of cell cycle kinetics based upon the flow cytometric detection of BrdU incorporation have replaced the cumbersome autoradiographic methodology utilizing tritiated thymidine. This unit distills the knowledge gained over this time into concise and easy to follow protocols for DNA content analysis following thermal denaturation or acid denaturation, as well as a modified method that combines thermal denaturation with washless immunocytochemical detection of BrdU.

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Year:  2001        PMID： 18770734     DOI： 10.1002/0471142956.cy0707s02

Source DB:  PubMed          Journal:  Curr Protoc Cytom        ISSN： 1934-9297


  15 in total

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