Literature DB >> 18770603

Genetic Manipulation of Mycobacterium tuberculosis.

Michelle H Larsen1, Karolin Biermann, Steven Tandberg, Tsugunda Hsu, William R Jacobs.   

Abstract

This unit includes protocols for the genetic manipulation of Mycobacterium tuberculosis, including nucleic acid extraction (plasmid DNA, genomic DNA, and mRNA), and methods for electroporation (transformation), transduction (including allelic exchange), and transposon mutagenesis. Considerations for working with M. tuberculosis at Biosafety Level 3 containment are also discussed. Copyright 2007 by John Wiley & Sons, Inc.

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Year:  2007        PMID： 18770603     DOI： 10.1002/9780471729259.mc10a02s6

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  83 in total

1.  Temperature-dependent regulation of mycolic acid cyclopropanation in saprophytic mycobacteria: role of the Mycobacterium smegmatis 1351 gene (MSMEG_1351) in CIS-cyclopropanation of alpha-mycolates.

Authors:  Laeticia Alibaud; Anuradha Alahari; Xavier Trivelli; Anil K Ojha; Graham F Hatfull; Yann Guerardel; Laurent Kremer
Journal:  J Biol Chem       Date:  2010-05-10       Impact factor: 5.157

2.  Polyphosphate is an extracellular signal that can facilitate bacterial survival in eukaryotic cells.

Authors:  Ramesh Rijal; Louis A Cadena; Morgan R Smith; Joseph F Carr; Richard H Gomer
Journal:  Proc Natl Acad Sci U S A       Date:  2020-12-02       Impact factor: 11.205

3.  Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories.

Authors:  Thomas R Ioerger; Yicheng Feng; Krishna Ganesula; Xiaohua Chen; Karen M Dobos; Sarah Fortune; William R Jacobs; Valerie Mizrahi; Tanya Parish; Eric Rubin; Chris Sassetti; James C Sacchettini
Journal:  J Bacteriol       Date:  2010-05-14       Impact factor: 3.490

4.  Effect of Antiretroviral Therapy on Treatment Outcomes in a Prospective Study of Extensively Drug-Resistant Tuberculosis (XDR-TB) HIV Coinfection Treatment in KwaZulu-Natal, South Africa.

Authors:  Katharine A Yuengling; Nesri Padayatchi; Allison Wolf; Barun Mathema; Tyler Brown; C Robert Horsburgh; Max R OʼDonnell
Journal:  J Acquir Immune Defic Syndr       Date:  2018-12-01       Impact factor: 3.731

5.  Coresistance to isoniazid and ethionamide maps to mycothiol biosynthetic genes in Mycobacterium bovis.

Authors:  Catherine Vilchèze; Yossef Av-Gay; S Whitney Barnes; Michelle H Larsen; John R Walker; Richard J Glynne; William R Jacobs
Journal:  Antimicrob Agents Chemother       Date:  2011-06-27       Impact factor: 5.191

6.  Identification of a glycosyltransferase from Mycobacterium marinum involved in addition of a caryophyllose moiety in lipooligosaccharides.

Authors:  Debasmita Sarkar; Mandeep Sidhu; Albel Singh; Jiemin Chen; David A Lammas; Astrid M van der Sar; Gurdyal S Besra; Apoorva Bhatt
Journal:  J Bacteriol       Date:  2011-03-04       Impact factor: 3.490

7.  Whole genome sequence analysis of multidrug-resistant Mycobacterium tuberculosis Beijing isolates from an outbreak in Thailand.

Authors:  Sanjib Mani Regmi; Angkana Chaiprasert; Supasak Kulawonganunchai; Sissades Tongsima; Olabisi Oluwabukola Coker; Therdsak Prammananan; Wasna Viratyosin; Iyarit Thaipisuttikul
Journal:  Mol Genet Genomics       Date:  2015-04-24       Impact factor: 3.291

8.  Selective Inactivity of Pyrazinamide against Tuberculosis in C3HeB/FeJ Mice Is Best Explained by Neutral pH of Caseum.

Authors:  Jean-Philippe Lanoix; Thomas Ioerger; Aimee Ormond; Firat Kaya; James Sacchettini; Véronique Dartois; Eric Nuermberger
Journal:  Antimicrob Agents Chemother       Date:  2015-11-16       Impact factor: 5.191

9.  The cell envelope-associated phospholipid-binding protein LmeA is required for mannan polymerization in mycobacteria.

Authors:  Kathryn C Rahlwes; Stephanie A Ha; Daisuke Motooka; Jacob A Mayfield; Lisa R Baumoel; Justin N Strickland; Ana P Torres-Ocampo; Shota Nakamura; Yasu S Morita
Journal:  J Biol Chem       Date:  2017-08-29       Impact factor: 5.157

10.  Genome analysis of multi- and extensively-drug-resistant tuberculosis from KwaZulu-Natal, South Africa.

Authors:  Thomas R Ioerger; Sunwoo Koo; Eun-Gyu No; Xiaohua Chen; Michelle H Larsen; William R Jacobs; Manormoney Pillay; A Willem Sturm; James C Sacchettini
Journal:  PLoS One       Date:  2009-11-05       Impact factor: 3.240
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