Literature DB >> 18770535

Use of potato virus X (PVX)-based vectors for gene expression and virus-induced gene silencing (VIGS).

Christophe Lacomme1, Sean Chapman.   

Abstract

Potato virus X-based vectors are a well established system for rapid in planta studies. The vectors can be used for expression of proteins in plants and to down-regulate genes through virus-induced gene silencing. The development of binary-based vectors for Agrobacterium delivery makes this system well suited to high-throughput studies. Protocols are given for establishing infections to achieve expression and VIGS through in vitro transcription and Agrobacterium delivery to glasshouse and in vitro-grown plant material.

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Year:  2008        PMID： 18770535     DOI： 10.1002/9780471729259.mc16i01s8

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  12 in total

1.  Dissecting the multifunctional role of the N-terminal domain of the Melon necrotic spot virus coat protein in RNA packaging, viral movement and interference with antiviral plant defence.

Authors:  Marta Serra-Soriano; José Antonio Navarro; Vicente Pallás
Journal:  Mol Plant Pathol       Date:  2016-08-14       Impact factor: 5.663

2.  Two light responsive WRKY genes exhibit positive and negative correlation with picroside content in Picrorhiza kurrooa Royle ex Benth, an endangered medicinal herb.

Authors:  Tanvi Sharma; Tabasum Kawoosa; Parul Gahlan; Damini Sharma; Anish Kaachra; Vipin Hallan; Sanjay Kumar
Journal:  3 Biotech       Date:  2020-05-16       Impact factor: 2.406

3.  A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize.

Authors:  Yu Mei; Chunquan Zhang; Bliss M Kernodle; John H Hill; Steven A Whitham
Journal:  Plant Physiol       Date:  2016-04-28       Impact factor: 8.340

4.  The destructive citrus pathogen, 'Candidatus Liberibacter asiaticus' encodes a functional flagellin characteristic of a pathogen-associated molecular pattern.

Authors:  Huasong Zou; Siddarame Gowda; Lijuan Zhou; Subhas Hajeri; Gongyou Chen; Yongping Duan
Journal:  PLoS One       Date:  2012-09-28       Impact factor: 3.240

5.  Development of a Mild Viral Expression System for Gain-Of-Function Study of Phytoplasma Effector In Planta.

Authors:  Sin-Fen Hu; Yu-Hsin Huang; Chan-Pin Lin; Li-Yu Daisy Liu; Syuan-Fei Hong; Chiao-Yin Yang; Hsiao-Feng Lo; Ting-Yu Tseng; Wei-Yao Chen; Shih-Shun Lin
Journal:  PLoS One       Date:  2015-06-15       Impact factor: 3.240

6.  Genome-Wide Identification, Expression Profile and Evolution Analysis of Karyopherin β Gene Family in Solanum tuberosum Group Phureja DM1-3 Reveals Its Roles in Abiotic Stresses.

Authors:  Ya Xu; Lu Liu; Pan Zhao; Jing Tong; Naiqin Zhong; Hongji Zhang; Ning Liu
Journal:  Int J Mol Sci       Date:  2020-01-31       Impact factor: 5.923

7.  Protein expression and gene editing in monocots using foxtail mosaic virus vectors.

Authors:  Yu Mei; Bliss M Beernink; Evan E Ellison; Eva Konečná; Anjanasree K Neelakandan; Daniel F Voytas; Steven A Whitham
Journal:  Plant Direct       Date:  2019-11-22

8.  Agrobacterium-Mediated Gene Transient Overexpression and Tobacco Rattle Virus (TRV)-Based Gene Silencing in Cassava.

Authors:  Hongqiu Zeng; Yanwei Xie; Guoyin Liu; Yunxie Wei; Wei Hu; Haitao Shi
Journal:  Int J Mol Sci       Date:  2019-08-15       Impact factor: 5.923

9.  Computational and biological characterization of fusion proteins of two insecticidal proteins for control of insect pests.

Authors:  Shaista Javaid; Sehrish Naz; Imran Amin; Georg Jander; Zaheer Ul-Haq; Shahid Mansoor
Journal:  Sci Rep       Date:  2018-03-19       Impact factor: 4.379

10.  Establishment of a novel virus-induced virulence effector assay for the identification of virulence effectors of plant pathogens using a PVX-based expression vector.

Authors:  Jinxia Shi; Yuanhong Zhu; Ming Li; Yuqing Ma; Huarong Liu; Peng Zhang; Di Fang; Yushuang Guo; Ping Xu; Yongli Qiao
Journal:  Mol Plant Pathol       Date:  2020-10-07       Impact factor: 5.663
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