Literature DB >> 18770531

Direct PCR of intact bacteria (colony PCR).

Michael E Woodman1.   

Abstract

This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.

Mesh：

Year:  2008        PMID： 18770531     DOI： 10.1002/9780471729259.mca03ds9

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  17 in total

1.  Oral colonization by Streptococcus mutans and caries development is reduced upon deletion of carbonic anhydrase VI expression in saliva.

Authors:  David J Culp; Bently Robinson; Seppo Parkkila; Pei-Wen Pan; Melanie N Cash; Helen N Truong; Thomas W Hussey; Sarah L Gullett
Journal:  Biochim Biophys Acta       Date:  2011-09-16

2.  Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing.

Authors:  Anna Paruzynski; Anne Arens; Richard Gabriel; Cynthia C Bartholomae; Simone Scholz; Wei Wang; Stephan Wolf; Hanno Glimm; Manfred Schmidt; Christof von Kalle
Journal:  Nat Protoc       Date:  2010-07-08       Impact factor: 13.491

3.  Deletion mutant library for investigation of functional outputs of cyclic diguanylate metabolism in Pseudomonas aeruginosa PA14.

Authors:  Dae-Gon Ha; Megan E Richman; George A O'Toole
Journal:  Appl Environ Microbiol       Date:  2014-03-21       Impact factor: 4.792

4.  Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells.

Authors:  Jeffrey A Fabrick; J Joe Hull
Journal:  J Vis Exp       Date:  2017-04-20       Impact factor: 1.355

5.  Borrelia burgdorferi cp32 BpaB modulates expression of the prophage NucP nuclease and SsbP single-stranded DNA-binding protein.

Authors:  Alicia M Chenail; Brandon L Jutras; Claire A Adams; Logan H Burns; Amy Bowman; Ashutosh Verma; Brian Stevenson
Journal:  J Bacteriol       Date:  2012-06-22       Impact factor: 3.490

6.  2-Heptyl-4-quinolone, a precursor of the Pseudomonas quinolone signal molecule, modulates swarming motility in Pseudomonas aeruginosa.

Authors:  Dae-Gon Ha; Judith H Merritt; Thomas H Hampton; James T Hodgkinson; Matej Janecek; David R Spring; Martin Welch; George A O'Toole
Journal:  J Bacteriol       Date:  2011-09-30       Impact factor: 3.490

7.  carP, encoding a Ca2+-regulated putative phytase, is evolutionarily conserved in Pseudomonas aeruginosa and has potential as a biomarker.

Authors:  Sergio E Mares; Michelle M King; Aya Kubo; Anna A Khanov; Erika I Lutter; Noha Youssef; Marianna A Patrauchan
Journal:  Microbiology (Reading)       Date:  2021-02       Impact factor: 2.777

8.  Deep Mutational Scanning of Protein-Protein Interactions Between Partners Expressed from Their Endogenous Loci In Vivo.

Authors:  Alexandre K Dubé; Rohan Dandage; Soham Dibyachintan; Ugo Dionne; Philippe C Després; Christian R Landry
Journal:  Methods Mol Biol       Date:  2022

9.  Spirosoma pulveris sp. nov., a bacterium isolated from a dust sample collected at Chungnam province, South Korea.

Authors:  Eun Sun Joo; Jae-Jin Lee; Seho Cha; Weonhwa Jheong; Taegun Seo; Sangyong Lim; Sun-Wook Jeong; Sathiyaraj Srinivasan
Journal:  J Microbiol       Date:  2015-10-28       Impact factor: 3.422

10.  SELMA: Selection with Modified Aptamers.

Authors:  J Sebastian Temme; Isaac J Krauss
Journal:  Curr Protoc Chem Biol       Date:  2015-06-01
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.