PURPOSE: Amphiphilic triblock copolymers such as the pluronic poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) L64 (pEO(13)-pPO(30)-pEO(13)) have been shown to mediate more efficient gene transfer in muscle as compared to naked DNA. We were interested in studying the effect of a chemical change of the central block of pluronic polymers on the transfection activity. METHODS: We synthesized new amphiphilic copolymers in which the hydrophobic pPO block was replaced by poly(tetrahydrofuran) (pTHF) chains. The resulting triblock pEO-pTHF-pEO polymers have been characterized by NMR and SEC and assayed for in vitro and in vivo gene transfer. RESULTS: The animal experiments showed that the new copolymers are able to significantly increase the transfection efficiency of plasmid DNA after intramuscular injection. CONCLUSIONS: These results indicate that the capacity to enhance plasmid DNA transfection in skeletal muscle is not restricted to pEO-pPO-pEO arrangements.
PURPOSE: Amphiphilic triblock copolymers such as the pluronic poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) L64 (pEO(13)-pPO(30)-pEO(13)) have been shown to mediate more efficient gene transfer in muscle as compared to naked DNA. We were interested in studying the effect of a chemical change of the central block of pluronic polymers on the transfection activity. METHODS: We synthesized new amphiphilic copolymers in which the hydrophobic pPO block was replaced by poly(tetrahydrofuran) (pTHF) chains. The resulting triblock pEO-pTHF-pEOpolymers have been characterized by NMR and SEC and assayed for in vitro and in vivo gene transfer. RESULTS: The animal experiments showed that the new copolymers are able to significantly increase the transfection efficiency of plasmid DNA after intramuscular injection. CONCLUSIONS: These results indicate that the capacity to enhance plasmid DNA transfection in skeletal muscle is not restricted to pEO-pPO-pEO arrangements.
Authors: Antoine Kichler; Christian Leborgne; Josefine März; Olivier Danos; Burkhard Bechinger Journal: Proc Natl Acad Sci U S A Date: 2003-01-31 Impact factor: 11.205
Authors: A James Mason; Christian Leborgne; Gilles Moulay; Amélie Martinez; Olivier Danos; Burkhard Bechinger; Antoine Kichler Journal: J Control Release Date: 2006-12-08 Impact factor: 9.776
Authors: L M Mir; M F Bureau; J Gehl; R Rangara; D Rouy; J M Caillaud; P Delaere; D Branellec; B Schwartz; D Scherman Journal: Proc Natl Acad Sci U S A Date: 1999-04-13 Impact factor: 11.205
Authors: O Boussif; F Lezoualc'h; M A Zanta; M D Mergny; D Scherman; B Demeneix; J P Behr Journal: Proc Natl Acad Sci U S A Date: 1995-08-01 Impact factor: 11.205