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Literature DB >> 18768972

Integration of rous sarcoma virus DNA: a CA dinucleotide is not required for integration of the U3 end of viral DNA.

Jangsuk Oh¹, Kevin W Chang, Stephen H Hughes.

Abstract

The two ends of RSV linear DNA are independently inserted into host DNA by integrase in vivo. We previously showed that the range of U3 sequences that are acceptable substrates for integrase appeared to be greater than the range of acceptable U5 sequences in vivo. We have done additional experiments to determine which U3 sequences are good integrase substrates. On the U3 end, there does not appear to be a stringent requirement for the canonical CA, integrase can efficiently remove three nucleotides, and six nucleotides are sufficient to allow integration with reasonable, albeit reduced, efficiency.

Entities: Chemical Species

Mesh：

Substances：
DNA, Viral
Integrases

Year: 2008 PMID： 18768972 PMCID： PMC2573277 DOI： 10.1128/JVI.01353-08

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

24 in total

1. DNase protection analysis of retrovirus integrase at the viral DNA ends for full-site integration in vitro.

Authors: A Vora; D P Grandgenett
Journal: J Virol Date: 2001-04 Impact factor: 5.103

2. Human immunodeficiency virus integrase protein requires a subterminal position of its viral DNA recognition sequence for efficient cleavage.

Authors: C Vink; D C van Gent; Y Elgersma; R H Plasterk
Journal: J Virol Date: 1991-09 Impact factor: 5.103

3. Structure of the termini of DNA intermediates in the integration of retroviral DNA: dependence on IN function and terminal DNA sequence.

Authors: M J Roth; P L Schwartzberg; S P Goff
Journal: Cell Date: 1989-07-14 Impact factor: 41.582

4. Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity.

Authors: P A Sherman; J A Fyfe
Journal: Proc Natl Acad Sci U S A Date: 1990-07 Impact factor: 11.205

5. The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro.

Authors: R Craigie; T Fujiwara; F Bushman
Journal: Cell Date: 1990-08-24 Impact factor: 41.582

6. Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer.

Authors: Mary Jane McWilliams; John G Julias; Stefan G Sarafianos; W Gregory Alvord; Edward Arnold; Stephen H Hughes
Journal: J Virol Date: 2003-10 Impact factor: 5.103

7. The effects of alternate polypurine tracts (PPTs) and mutations of sequences adjacent to the PPT on viral replication and cleavage specificity of the Rous sarcoma virus reverse transcriptase.

Authors: Kevin W Chang; Jangsuk Oh; W Gregory Alvord; Stephen H Hughes
Journal: J Virol Date: 2008-06-18 Impact factor: 5.103

8. Activities of human immunodeficiency virus (HIV) integration protein in vitro: specific cleavage and integration of HIV DNA.

Authors: F D Bushman; R Craigie
Journal: Proc Natl Acad Sci U S A Date: 1991-02-15 Impact factor: 11.205

9. Using pyrrolo-deoxycytosine to probe RNA/DNA hybrids containing the human immunodeficiency virus type-1 3' polypurine tract.

Authors: Chandravanu Dash; Jason W Rausch; Stuart F J Le Grice
Journal: Nucleic Acids Res Date: 2004-03-05 Impact factor: 16.971